Planothidium lacustre (Achnanthidiaceae), first world record outside the type locality

Silver nanoparticles were synthesised using silver nitrate and tri-sodium citrate and were functionalized with 2,5-Dimercapto-1,3,4-thiadiazole (DMTD) linking molecules. Functionalized silver nanoparticles dispersed within 4-(trans-4-n hexylcyclohexyl) isothiocyanatobenzoate (6CHBT) liquid crystal (LC) matrices have attracted significant attention because of their potential for tailoring tuneable surface plasmonic, optical, and electronic properties by controlling the self-assembly of silver dots. The unique combination of functionalized nanoparticles and 6CHBT LCs offers a versatile platform for manipulating interactions at the nanoscale. The dispersion of functionalized Ag nanoparticles within the LCs appears to substantially modify the plasmonic and opto-electronic properties of the Ag QDs. The formation and morphology of the self-assembled structures of silver nanoparticles were confirmed by Optical and Scanning Electron Microscopy. Optical microscopy (OM) and Scanning Electron Microscopy (SEM) images of Ag-doped 6CHBT confirmed the formation of nano-micro spheres, as well as ring and rod-like structures in LC media. We have used High-Resolution X-ray diffraction (HR-XRD), Fourier-Transform Infrared Spectroscopy (FTIR), Surface-Enhanced Raman Spectroscopy (SERS), Optical Microscopy (OM), Scanning Electron Microscopy (SEM), Ultraviolet-visible-near-infrared Spectroscopy (UV-Vis-NIR), and Fluorescence (FL) Spectroscopy. We calculated the electronic properties such as the bandgap of the synthesised Ag QDs and functionalized Ag QDs using UV-Vis-NIR and Tauc’s plot curves. Surface-enhanced Raman spectroscopy, Optical microscopy, SEM, and UV-Vis-NIR spectral data were used to correlate the properties of the LC and QDs hybrid systems.

The Author(s) 2009. This article is published with open access at Springerlink.com Abstract Temporary ponds are seasonal wetlands annually subjected to extreme and unstable ecolog- ical conditions, neither truly aquatic nor truly terres- trial. This habitat and its flora have been poorly studied and documented because of the ephemeral character of the flora, the changeable annual weather that has a great effect on the small, herbaceous taxa and the declining abundance of temporary ponds. The objectives of this study are: (a) to define plant community diversity in terms of floristic composition of ephemeral wetlands in SW Portugal, (b) to identify temporary pond types according to their vegetation composition and (c) to identify those ponds that configure the European community priority habitat (3170* - Mediterranean temporary ponds). Vegetation sampling was conducted in 29 ponds, identifying 168 species grouped among 15 plant communities. Soil texture, pH, organic C and N content were measured, but only N and percent of clay appear to be related with the distribution of each community type. The results showed that ephemeral wetlands could be classified into four type: vernal pools, marshlands, deep ponds and disturbed wet- lands. Vernal pools correspond to the Mediterranean temporary ponds (3170*), protected as priority hab- itat under the EU Habitats Directive. Submersed Isoetes species (Isoetes setaceum and Isoetes vela- tum) represents, together with Eryngium cornicula- tum, the indicator species for vernal pools. We identify also indicator plant communities of this priority habitat, namely I. setaceum and E. cornicul- atum-Baldellia ranunculoides plant communities. In this region, the conservation of temporary ponds has so far been compatible with traditional agricultural activities, but today these ponds are endangered by the intensification of agriculture and the loss of traditional land use practices and by the development of tourism.

Aquatic environmental DNA is increasingly used for biodiversity monitoring, such as surveying threatened and invasive species. Mainstreaming these methods in practical applications, however, still requires significant standardization and optimisation, namely regarding DNA capture methods. Here we evaluated how filter type (standard disc filters vs high‐capacity capsules), number of sampling sites, volume of water filtered and environmental factors affected amphibian detection in Mediterranean temporary ponds. The study involved water filtering until clogging at one (capsules) and five (discs) sites from 16 small and shallow ponds, where three urodele and seven anuran species were recorded through sweep‐netting and adult observations. Detection probabilities were estimated from site occupancy models based on replicate sampling and from an adaptation of time‐to‐detection models relating detection probability to volume of water filtered. Discs filtered relatively small volumes (15–1250 mL), with detection probabilities of the two abundant species (Pelobates cultripes, Hyla meridionalis) increasing rapidly with sample size and water volume, reaching almost perfect detection (0.95) at four and seven discs, and 420 mL and 1860 mL, respectively. However, reaching high detection probabilities for rare species (Pelodytes atlanticus, Pleurodeles waltl, Triturus pygmaeus) would require larger sampling effort than that used in our study. Despite filtering much larger volumes (600–5300 mL), filtering with capsules at a single site per pond provided lower detection probabilities for abundant species than filtering with discs at five sites. Rarer species showed no difference between methods, which may be due to small sample sizes and reduced statistical power for species with few detections. The effect of conductivity on species detectability was largely negative, while the influence of water clarity varied across species, and pH had no effects. Overall, our results suggest that eDNA amphibian surveys in Mediterranean temporary ponds need to consider filter clogging, heterogeneous DNA distribution, and highly conductive waters.

Mediterranean temporary ponds (MTPs) are highly distinctive habitats with intermittent and unstable environmental conditions, inhabited by several rare and endemic plant and animal species. Temporary pond ecosystems have been classified as endangered all over the world; nevertheless, they are disappearing at a high rate, especially in the Mediterranean region. MTPs are a priority habitat under the European Union Habitats Directive and thus appropriate management and conservation efforts should be applied. Further research is needed in order to better understand the environmental characteristics and ecology of this habitat which is not always the case in many countries. In this study a range of environmental data were collected from the Greek MTP habitat sites including the altitude, the geology, the representativity, the ecological status and other biological data aiming to assess the environmental status of all known Greek MTPs. Statistical analysis were performed and thematic maps were created using a Geographical Information System (GIS). Greek MTP’s were classified according to their characteristics and habitat pressures were identified and further analysed. A comparison between the present environmental status of the MTPs in Greece with the MTPs in other Mediterranean countries was undertaken and relevant conservation and management measures were proposed. The outcome of this study was that a significant percentage of the MTP habitat sites in Greece are at risk mainly due to human activities, such as agricultural pollution, expansion of cropland and water resources over-exploitation. It was concluded that immediate restoration and conservation actions should be taken so as to prevent further ecological degradation.

<p>Mediterranean temporary ponds are very shallow ponds, isolated from permanent water bodies, which undergo a periodic cycle of flooding and drought, and have a characteristic flora and fauna adapted to this alternation. This habitat is mainly distributed in dry and sub-arid areas. Mediterranean temporary ponds are identified as one of the worldwide biodiversity hotspots and constitutes therefore a priority habitats according to the Natura 2000 network of the European Union (3170*, Council Directive 92/43/CEE). The development of flora and fauna in this type of ecosystem is defined by the natural length of the hydro-period. However, little is known about the hydrological functioning of these very specific hydrosystems. DespiteHS10 this protective conservation status, this habitat has suffered continuous degradation and loss disappearing at a fast rate due anthropogenic impacts and climate pressures. In most cases, temporary wetland disappearance is unintentional and related to a lack of understanding of its hydrological functioning within the watershed.</p><p>The aim of this work is, hence, to use the tools of the isotope hydrology to increase our basic understanding of the hydrological functioning of the Mediterranean temporary ponds. Our study focuses on the Musella temporary pond located in Southern Corsica (France) which undergoes important man-induced and climatic pressures. During one full hydrological cycle, surface and groundwater levels, major ions, stable isotopes of the water molecules as well as field parameters (temperature, pH, electrical conductivity, dissolved oxygen) have been measured every month.</p><p>Results bring information on the water quality, chemical stability and temporal evolution in terms of surface water level as well as potential connection with the underlying carbonated aquifer. The stable isotopes inform about the origin of water, its mixing processes with groundwater, and its evaporative status through time.</p><p>Flooding and drying processes of the Musella temporary pond are now better constrained and documented projections can now be set up towards the resilience of the hydrosystem considering the future consequences of climate change in the Mediterranean region.</p>

Mediterranean Temporary Ponds (MTPs) constitute priority habitat under the European Union Habitats’ Directive. They are inhabited by rare species and subjected to unstable environmental conditions. Lakes and ponds act as early indicators of climate change, to which high altitude ecosystems are especially vulnerable. This study presents a full dataset of the geo-environmental parameters of such habitats (MTPs) along with their current ecological status for the first time. Furthermore, this paper aims to address the lack of basic geo-environmental background on the network of MTPs of Mt. Oiti concerning their geological, geomorphological, mineralogical and geochemical characteristics along with the pressures received from various activities. The study area is located in a mountainous Natura 2000 site of Central Greece, which hosts four MTPs. Fieldwork and sampling of water and bottom sediments were carried out during dry and wet periods between 2012 and 2014. Electrical Resistivity Tomography measurements identified synforms shaped under the ponds that topography does not always adopt them, mostly due to erosion procedures. The most significant feature, distinguishing those pond waters from any other province water bodies is the extremely low content of all studied ions (including NO2−, NO3−, NH4+, PO43−, HCO3−, SO42−, Al, As, B, Ba, Ca, Cd, Ce, Cl, Co, Cr, Cs, Cu, Fe, Ga, Gd, Ge, Hf, Hg, K, La, Li, Mg, Mn, Mo, Na, Ni, P, Rb, S, Sb, Se, Si, Sn, Sr, Ti, U, V, W, Zn, and Zr). MTPs water bodies are of bicarbonate dominant type, and a fresh meteoric water origin is suggested. The main pressures identified were grazing and trampling by vehicles. MTPs of Mt. Oiti were classified according to their ecological status form excellent to medium. Our results can contribute to a better understanding of the mountainous temporary ponds development in the Mediterranean environment.

Microscopy tools for product innovation

Palo Laziale Wood is a relatively small biotope (129 ha) situated along the coast of the Metropolitan Area of Rome, Lazio region, Italy. Despite being one of the region's remaining patches of an ancient oak floodplain broad-leaved forest, it conserves numerous priority habitats and species of high conservation interest. The vegetation consists mainly of Turkey oak stands with small temporary ponds and flooded meadows. The forest underwent a dieback in 2003, triggered by a significantly hot and dry summer. In 2018, an ecological restoration project (LIFE PRIMED LIFE17 NAT/GR/000511) was initiated to restore Palo Laziale’s ecosystems. This paper presents the methodological approach employed to assess the vegetation ecology of a degraded forest ecosystem. Such an investigation was a key component of the Adaptative Restoration Plan of the Project. It provided the baseline necessary for designing and calibrating the planned direct conservation actions on the target habitat types (91M0: Pannonian-Balkanic turkey oak-sessile oak forests, 3170*: Mediterranean temporary ponds, 5230*: Arborescent matorral with Laurus nobilis, etc.) and establishing reference values to enable long-term monitoring. Plant taxa comparison from 1 ha square-grid sampling and multivariate analyses were carried out to group species and identify environmental and Ellenberg-based drivers. Six ecologically distinct units were found, eventually confirming the distinctive ecological heterogeneity of Mediterranean ecosystems. Amongst these, the hygrophilous vegetation has resulted to be the one mainly affected by the dieback outbreak. Due to the high heterogeneity, introduced by the massive tree mortality, the method of regular 1 ha squares turned out to be a reliable alternative to random vegetation sampling plots (e.g., phytosociological relevés) to disentangle ecological patterns of fragmented and disturbed habitats.

The article presents the results of measurements of the principal ions content, electrical conductivity and pH for 13 lakes and small (temporary) water ponds in the east part of the Thala Hills, Enderby Land, East Antarctica. Water sampling was carried out by participants of the seasonal Belarusian Antarctic expeditions in the period from 2011 to 2018. The purpose of the study is the evaluation of the hydrochemical composition of lakes and temporary ponds of the Thala Hills (on an example of the Vecherny Oasis), identification of natural and anthropogenic factors which determine the variability of the hydrochemical parameters for assessment of vulnerability of lakes and temporary ponds to anthropogenic impacts and climate change. It is shown that the waters of the lakes of this region are low mineralized with the sum of ions within the range of 10.6–87.5 mg/l (the average is 34.5 mg/l), electrical conductivity — 19.3–130.0 μS/cm (61.3 μS/cm). The water is characterized as slightly acidic and neutral. The waters of small (temporary) ponds are characterized by greater variability of hydrochemical parameters in comparison with lakes: the sum of ions is in the range of 6.7–915.0 mg/l (the average is 158.0 mg/l), the electrical conductivity is 4.6–1663.0 μS/cm (the average is 267.0 μS/cm). Coefficients of variation for most compounds in the waters of temporary ponds exceed 100 %. In most cases the predominance of sodium and chloride ions was established, which indicates the influence of marine aerosols on chemical composition of water lakes and temporary ponds. Elevated concentrations of mineral elements in the water of temporary ponds are caused by the lack of flow and, and as a consequence of thies, the accumulation of salts as a result of evaporation.

Temporary ponds represent a specific type of ecosystem extensively widespread worldwide. They are better known as copular pools, ephemeral waters, karst sinkholes, seasonal wetlands, and vernal pools. Among these, Mediterranean Temporary Ponds (MTPs) represent a priority habitat according to the Natura 2000 network of the European Union. Their main characteristic is represented by their depth of only a few centimeters and lack of communication with permanent water bodies. MTPs habitats are vulnerable to human activities, especially agriculture, and they are considered priority habitats to safeguard. Threats affecting this habitat are various and many and depend on specific site conditions, including intensive agriculture, tree planting, abandonment of traditional land use, and excessive grazing. In the present manuscript, we report the results of monitoring activity of some of these sites in Southern Italy aimed at understanding the ecological status of these ephemeral ecosystems with a specially developed methodology based on data integration.

Conservation of Mediterranean temporary ponds under agricultural intensification: an evaluation using amphibians

Mediterranean temporary ponds are recognized as conservation priority habitats that face anthropogenic threats and are important habitats for a number of aquatic and terrestrial animals and plants. Bats are a diverse group of animals that use ponds for drinking and feeding on emerging aquatic insects and terrestrial insects in the riparian zone. We investigated the importance of temporary ponds for bats in Greece by acoustically sampling bat community structures and activity at temporary ponds throughout the year. We sampled monthly, from 3 to 13 months in 2019–2020, at sites at the pond edge and approximately 150–300 m away from the edge, at four temporary ponds in northern and southern Greece. Our results confirm the importance of temporary ponds for bats as activity was recorded year-round and was high in all but the winter months. In general, the distance to the edge of the pond and the presence of water in the pond explained bat activity together with air temperature. Importantly, whether dry or not, all ponds supported bat activity, independent of their particular characteristics. This study highlights the urgent need for the conservation of temporary ponds, especially in areas with limited water availability.

Mediterranean temporary ponds are present in five biogeographical regions, which are characterized by hot dry summers and cool mild winters. These ponds host high levels of biodiversity and endemism. More than 200 families and 630 genera of invertebrates have been reported, including iconic large branchiopods. Mediterranean climates show strong temporal variability, reflected in large intra- and inter-annual changes in pond hydroperiod that drive patterns in invertebrate communities. Consequently, community dynamics are more temporally variable than in cold-temperate temporary ponds. Globally, temporary pond conservation has been neglected and many ponds have already been lost despite the presence of legal protections and high ecological importance. Future biodiversity protection requires mosaics of ponds with diverse hydroperiods, preserving natural hydroregimes.KeywordsInvertebrates, temporary ponds, Mediterranean regions, seasonal succession, life‑histories, predation, conservation

Temporary ponds (TPs) are shallow water bodies characterized by alternating phases of drought and flooding. They exhibit a small-scale zonation with a central belt (CB), an intermediate belt, and an outer belt (OB). Starting from the hypothesis that plant assemblage composition is affected by the position within TPs, our aims were to define the small-scale patterns of plant functional types, soil evolution, and soil physicochemical properties and to analyze their relationships and how they were affected by pond characteristics (i.e., elevation, substratum, total size, belt size, maximum water depth, and hydroperiod). Our results pointed out that patterns of plant functional types and soil evolution followed the position within the pond, soil physicochemical properties were weakly affected, and there was a relationship between plant functional types, soil small-scale patterns, and pond characteristics. Principal component analysis showed a positive correlation of Alfisols with CB, aquatic and amphibious species, maximum water depth, and hydroperiod, and of Entisols with OB and terrestrial species. A combined microtopography–hydrology effect was the primary factor controlling soil evolution and plant functional type patterns. As a consequence, modification in topographic shape and/or hydrologic parameters could alter TP environments. The results provide information for management strategies as an answer to the concerns surrounding continued worldwide TP decline.

Exploring the utility of optical microscopy versus scanning electron microscopy for the quantification of dental microwear