Abstract
Protein incorporated later into tight junctions (Pilt), also termed tight junction-associated protein 1 or tight junction protein 4, is a coiled-coil domain-containing protein that was originally identified as a human discs large-interacting protein. In this study, we identified Pilt as an Arf6-binding protein by yeast two-hybrid screening. By immunocytochemical analysis, Pilt was shown to be predominantly localized at the trans-Golgi complex and to exhibit diffuse cytoplasmic distribution in association with endosomes and plasma membrane in NIH3T3 cells. Silencing of endogenous Pilt disrupted the Golgi structure. The present findings suggest the functional involvement of Pilt in the maintenance of the Golgi structure. Structured summary of protein interactionsGM130 and Piltcolocalize by fluorescence microscopy (View interaction)Arf6(Q67L)physically interacts with Pilt by two hybrid (View Interaction: 1, 2)Piltphysically interacts with Arf6(Q67L) by pull down (View interaction)
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