Abstract
We present the results of picosecond time-resolved absorption spectroscopy of luciferin, substrate of the bioluminescence reaction. Both absorption and emission from the excited states contribute to the detected signal. Time evolution of the differential optical density at different wavelengths is analyzed. Characteristic rate constants of the relaxation processes are determined. The possible relationship between the behavior of the luciferin molecule in the excited state and the properties of the luciferin–luciferase complex is discussed.
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