Abstract
Phytochemical screening and allelopathic evaluation of aqueous and methanolic leaf extracts of Populus nigra L.
Highlights
Allelopathic bioassay demonstrated that leaf extracts had detrimental influence on the seed germination of four cultivars of wheat, which depended on the amount of powder dissolved in extracts (10, 20, 40 and 80 g)
Phytochemical evaluation of the aqueous leaf extracts of P. nigra revealed that secondary metabolites such as phenols, alkaloids, flavonoids, steroids, terpenoids, saponins, tannins, glycoside, reducing sugars, triterpenes and phytosterols were present in the samples
Significantly lower germination of wheat cultivars in both aqueous and methanolic extracts may be attributed to the presence of phytotoxic chemicals as we identified the presence of phenols, alkaloids, tannins, glycosides, reducing sugar, saponins, carbohydrates, steroids, terpenoids, reducing sugars, triterpenes, phytosterols, and proteins in sample extracts
Summary
These compounds are supposed to play an important role in defense and interactions of producers with receivers. Aqueous and methanolic leaf extracts of Populus nigra were screened for the presence of secondary metabolites and tested for the allelopathic effects on seed germination of four wheat cultivars (Ghazanvi, Siran, Atta Habib and Janbaz). Phytochemical screening revealed the presence of alkaloids, flavonoids, steroids, terpenoids, saponins, tannins, phlobatanins, and glycoside, reducing sugars, triterpenes, phytosterols and proteins in leaf extracts. The aim of the present work was to carryout phytochemical screening of aqueous and methanolic leaf extracts of P. nigra and to test them on germination of wheat. Alkaloids test Crude extracts (0.5 g) were warmed with 5 ml of 2% H2SO4 for 2 minutes, filtered and 2-3 drops of Dragendrof’s reagent was added to each filtrate and observed for red precipitation. Least significant difference test was used to record the effect of extracts on germination % of wheat cultivars
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