Abstract
The aim of study was to validate, by in vitro and in vivo studies, the traditional use for wound-healing activity of Artemisia absinthium L. Reversed-phase liquid chromatography coupled with diode array detection and electrospray ion trap mass spectrometry (RP-LC-DAD-ESI-MS) analysis allowed to identify eleven polyphenols with chlorogenic acid as the most abundant compound (3.75 g/100 g of dry extract). After that, antibacterial activity as well as acute dermal and oral toxicity were assessed in animal models. In order to investigate the wound-healing activity of A. absinthium methanol extract, two ointments were formulated (MEO 5% and 10%). The ointment with the highest concentration of plant extract (10%) showed a statistically significant effect on the rats wound contraction, similar to that exerted by the reference drug Cicatryl-Bio. Moreover, A. absinthium methanol extract showed the best antibacterial activity against the Gram-negative Escherichia coli ATCC 10536 (MIC 1.25–2.5 mg/mL) and the Gram-positive Staphylococcus aureus ATCC 6538 (0.31–0.625 mg/mL). The absence of oral and topical toxicity of the treated animals allowed to establish the safety of the ointments. Overall, data collected in the present study support and validate the use of A. absinthium as a wound healing agent in the Algerian traditional medicine.
Highlights
There is a growing interest on the importance of medicinal plants and traditional practices in solving the world health care problems
The extraction yield of methanol extract from the flowering aerial parts of A. absinthium collected in Algeria was 20.85 ± 0.84% w/w
The preliminary phytochemical screening carried out by different in vitro assays, able to identify different class of compounds, showed that methanol extract contains a high amount of total phenols, 180.33 ± 16.25 mg gallic acid equivalents (GAE)/g of dry extract (DE)
Summary
There is a growing interest on the importance of medicinal plants and traditional practices in solving the world health care problems. Because of this awareness, the international trade in medicinal plants is growing exponentially and many countries have assimilated traditional medical practices as an integral part of their culture [1]. The number of taxa of its flora is estimated to be about 4000, including 300 endemic taxa of which approximately 90% are present in the north of the country [2]. Notwithstanding this large patrimony of Algerian flora, only few species have been deepen investigated. Asteraceae represent one of the most important families of medicinal and aromatic plants growing in Algeria and comprises, among others, nine species of Artemisia [3]
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