Phytochemical profile, anti-oxidant and hepatoprotective activity Ocimum tenuiflorum leaves against carbon tetrachloride–induced hepatotoxicity in Mice

Abstract

Ocimum tenuiflorum is a medicinal plant with therapeutic potential, belongs to the family Lamiaceae also known as, tulsi and is known for its anti-oxidant and hepatoprotective activity. I have been selected groups were divided into two different doses, test groups (200 and 400 mg/kg) of the crude extracts, the standard drug (silymarin 100 mg/kg), and the hepatotoxicant carbone tetrachloride was negative control. The result of anti-oxidant and hepatoprotective activity suggested that effects of Ocimum tenuiflorum leaves extract were tested on phytochemical, results showed the presence of alkaloid, saponins, steroid, phenolic compounds flavonoids, tannins as chemical constituents may have anti-oxidant and hepatoprotective activity, which is not evaluated till now.Amoung the results, shows The amount of steroids was estimated to be 11.85 mg/g and 57.69 mg/g respectively for methanol extracts respectively. The amount of alkaloids was estimated to be 121.69 mg/g and 12.30 mg/g respectively for methanol extracts respectively. The amount of phenolic compounds was estimated to be 71.32 mg/g for methanol extract. The amount of flavonoids was estimated to be 116.60 mg/g respectively for methanol extracts respectively. The IC50 concentration of methanol extract was noticed to be very close to standard suggest that the activity of methanol extract was very high. The 80% methanol extract decreased the absolute and relative weight of the liver of mice at the doses of 200 and 400 mg/kg (p< 0.01 and p< 0.001, respectively). It also suppressed the plasma levels of AST and ALT (p< 0.001) in the aforementioned doses. Among extracts, the methanol fraction showed maximum hepatoprotective activity in its dose of 400 mg/kg (p< 0.001, in all cases). Ocimum tenuiflorum is endowed with hepatoprotectiveactivity, probably mediated via its antioxidant and anti-inflammatory activity. Thus, Ocimum tenuiflorum can be taken as one candidate for the development of hepatoprotective agents because of its good safety profile.