Phytochemical Identification and in vitro Radical Scavenging Activities of Medicated Butter Milk “Gauri Takra”: The Ancient Traditional Recipe of Ayurveda

The hawthorn Crataegus mexicana is a traditional Mexican fruit with properties that make this fruit useful for the treatment of many ailments, including diseases of the respiratory and urinary tract. This paper reports the antioxidant capacity of the n-hexane, dichloromethane, ethyl acetate, acetone, ethanol and methanol extracts of C. mexicana. Samples were evaluated for total phenolic and carotenoid contents, 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging, the inhibition of the formation of thiobarbituric acid reactive species (TBARS) and the neutralization of the cation-radical 2,2´-azino-bis(3ethyl benzothiazoline-6-sulfonic acid) diammonium salt (ABTS). The total phenolic content was 2.65 ± 0.23 mg of gallic acid equivalents per gram, and the carotenoid content was 26.4 ± 0.02 µg/g in dry hawthorn skin. The most active extract in scavenging DPPH radicals and inhibiting TBARS formation was the acetone extract, with activities of 21.9 ± 0.15 and 13.27 ± 0.70%, respectively, at 10 mg/L. The extracts were compared for activity against ascorbic acid, caffeic acid, α- tocopherol and quercetin. The acetone extract was the most active, with an IC50 value of 15.2 mg/L in DPPH and 17.7 mg/L in TBARS. A high correlation was observed between the results for TBARS and DPPH. These results demonstrate the potential nutritional and antioxidant value of this Mexican fruit.

Medicinal plants are an essential reservoir of natural compounds with diverse pharmacological properties. This study focuses on Debregeasia saeneb, (Forsk.) Hepper & J.R.I.Wood a relatively unexplored plant recognized for its traditional medicinal uses. The research aims to identify and analyze the phytochemical composition of D. saeneb (Forsk.) Hepper & J.R.I.Wood leaves, as well as evaluating their antioxidant and antidiabetic potential. This study systematically identified and analyzed the phytochemical constituents of D. saeneb (Forsk.) Hepper & J.R.I.Wood leaves utilizing Gas Chromatography-Mass Spectrometry (GC-MS), alongside both quantitative and qualitative assays. The biological activities were assessed through radical scavenging assays, specifically DPPH (1,1-di-phenyl-2-picrylhydrazyl), ABTS (2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), and α-amylase inhibitory assays, which have been largely underexplored across various solvents. The GC-MS analysis revealed a diverse range of compounds, with ethyl acetate extracts containing six compounds, chloroform extracts containing seven, and n-hexane extracts containing three distinct compounds. Further qualitative assessments confirmed the presence of glycosides, leucoanthocyanins, quinones, lignins, carbohydrates, terpenoids, anthraquinones, and phlobatannins in the extracts of D. saeneb (Forsk.) Hepper & J.R.I.Wood. Quantitative analyses established the total phenolic, tannin, and flavonoid contents, revealing a positive correlation among the different extracts. Specifically, the total flavonoid (TF) content in the standard ranged from 0.34 to 1.189 µg/mL, while in methanol extracts it ranged from 0.087 to 0.778 µg/mL, in ethyl acetate from 0.188 to 0.624 µg/mL, and in n-hexane from 0.03 to 0.44 µg/mL. The total tannin content (TTC) in the standard ranged from 0.462 to 2.359 µg/mL, with methanol extracts showing values from 0.016 to 0.048 µg/mL, ethyl acetate from 0.0196 to 0.153 µg/mL, and n-hexane from 0.0012 to 0.134 µg/mL. The total phenolic content (TPC) in the standard ranged from 0.11 to 0.38 µg/mL, with methanol extracts ranging from 0.042 to 0.267 µg/mL, ethyl acetate from 0.0275 to 0.487 µg/mL, and n-hexane from 0.001 to 0.348 µg/mL. In ethanol extracts, the radical scavenging capacity (RSC) ranged from 0.005 to 0.05 µg/mL, in methanol from 0.01 to 0.09 µg/mL, and in aqueous extracts from 0.005 to 0.035 µg/mL, while the standard RSC ranged from 0.5 to 4.5 µg/mL. The biological activity assays indicated that D. saeneb (Forsk.) Hepper & J.R.I.Wood extracts exhibited significant α-amylase inhibition, suggesting potential anti-diabetic properties. Furthermore, all extracts demonstrated radical scavenging activity in both ABTS and DPPH assays, with the methanol extract exhibiting the highest antioxidant activity. In conclusion, this research provides comprehensive insights into the phytochemical profile of D. saeneb (Forsk.) Hepper & J.R.I.Wood leaves and its potential therapeutic applications, including anti-diabetic and antioxidant effects, thereby warranting further investigation into molecular mechanisms, drug development, and health promotion.

Medicinal plants are an essential reservoir of natural compounds with diverse pharmacological properties. This study focuses on Debregeasia saeneb, (Forsk.) Hepper & J.R.I.Wood a relatively unexplored plant recognized for its traditional medicinal uses. The research aims to identify and analyze the phytochemical composition of D. saeneb (Forsk.) Hepper & J.R.I.Wood leaves, as well as evaluating their antioxidant and antidiabetic potential. This study systematically identified and analyzed the phytochemical constituents of D. saeneb (Forsk.) Hepper & J.R.I.Wood leaves utilizing Gas Chromatography-Mass Spectrometry (GC-MS), alongside both quantitative and qualitative assays. The biological activities were assessed through radical scavenging assays, specifically DPPH (1,1-di-phenyl-2-picrylhydrazyl), ABTS (2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), and α-amylase inhibitory assays, which have been largely underexplored across various solvents. The GC-MS analysis revealed a diverse range of compounds, with ethyl acetate extracts containing six compounds, chloroform extracts containing seven, and n-hexane extracts containing three distinct compounds. Further qualitative assessments confirmed the presence of glycosides, leucoanthocyanins, quinones, lignins, carbohydrates, terpenoids, anthraquinones, and phlobatannins in the extracts of D. saeneb (Forsk.) Hepper & J.R.I.Wood. Quantitative analyses established the total phenolic, tannin, and flavonoid contents, revealing a positive correlation among the different extracts. Specifically, the total flavonoid (TF) content in the standard ranged from 0.34 to 1.189 µg/mL, while in methanol extracts it ranged from 0.087 to 0.778 µg/mL, in ethyl acetate from 0.188 to 0.624 µg/mL, and in n-hexane from 0.03 to 0.44 µg/mL. The total tannin content (TTC) in the standard ranged from 0.462 to 2.359 µg/mL, with methanol extracts showing values from 0.016 to 0.048 µg/mL, ethyl acetate from 0.0196 to 0.153 µg/mL, and n-hexane from 0.0012 to 0.134 µg/mL. The total phenolic content (TPC) in the standard ranged from 0.11 to 0.38 µg/mL, with methanol extracts ranging from 0.042 to 0.267 µg/mL, ethyl acetate from 0.0275 to 0.487 µg/mL, and n-hexane from 0.001 to 0.348 µg/mL. In ethanol extracts, the radical scavenging capacity (RSC) ranged from 0.005 to 0.05 µg/mL, in methanol from 0.01 to 0.09 µg/mL, and in aqueous extracts from 0.005 to 0.035 µg/mL, while the standard RSC ranged from 0.5 to 4.5 µg/mL. The biological activity assays indicated that D. saeneb (Forsk.) Hepper & J.R.I.Wood extracts exhibited significant α-amylase inhibition, suggesting potential anti-diabetic properties. Furthermore, all extracts demonstrated radical scavenging activity in both ABTS and DPPH assays, with the methanol extract exhibiting the highest antioxidant activity. In conclusion, this research provides comprehensive insights into the phytochemical profile of D. saeneb (Forsk.) Hepper & J.R.I.Wood leaves and its potential therapeutic applications, including anti-diabetic and antioxidant effects, thereby warranting further investigation into molecular mechanisms, drug development, and health promotion.

Moringa stenopetala is a socioeconomic valued tree that is widely available and cultivated in Southern part of Ethiopia. The leaves have been traditionally used as a food source with high nutritional and medicinal values. The present work was carried out to evaluate the effect of thermal treatment on the total phenolic content, total flavonoid content, antioxidant activities and α-amylase inhibition of aqueous leaf extracts obtained from M. stenopetala during maceration and different decoction time interval (5, 10 and 15 min). The total phenolic and flavonoid contents were determined by the Folin-ciocalteu and aluminum chloride methods, respectively whereas antioxidant activities were determined by 2,2-diphenyl-1-picryl-hydrazyl(DPPH) radical scavenging, reducing power, phosphomolybdenum and ferrous ion chelating assays and α-amylase inhibition potential was determined using 3,5-dinitrosalicylic acid method. Total phenolic and total flavonoid contents ranged from 34.35 ± 1.06 to 39.47 ± 1.33 mgGAE/g and 10.44 ± 0.61 to 20.36 ± 0.93 mgQRE/g, respectively. Decoction for 10 min extract showed ferrous ion chelating (92.52 ± 0.17 %), DPPH radical scavenging (91.52± 0.59 %), α-amylase inhibition (69.06 ± 0.14%), ferric reducing power (0.765 ± 0.14) and total antioxidant activity (0.329 ± 0.32), respectively. DPPH, reducing power, total antioxidant and α-amylase inhibition activities showed positive linear correlation (R2=0.853, R2= 0.857 , R2= 0.864 and R2=0.930), respectively with total phenolic content but ferrous ion chelating activity were found to be weakly correlated (R2=0.481). Based on present investigation, it could be concluded that major lose of total phenolic content, antioxidant and α-amylase inhibition activities of the crude leaf extracts of M. stenopetala leaves were observed at decoction time for 15 min. Therefore, to maintain the total phenolic content, antioxidant and α-amylase inhibition activities of leaves, cooking practice should be at the optimum decoction time (5-10 min). Key words: Moringa stenopetala, antioxidant, total phenolic content, α-amylase inhibition.

The study aimed to investigate bioactive phytochemicals and antioxidant activity of Elaeocarpus tectorius (Lour.) Poir leaf extract. The antioxidant properties were evaluated by four antioxidant methods, 2,2-diphenyl-1-picryl-hydrazyl-hydrate assay, hydroxyl radical (HO%) scavenging assay, superoxide anion (O2%) radical scavenging assay, and nitric oxide (NO%) scavenging assay. The free radical scavenging activity of hydroethanolic extract of E. tectorius was found to be high for DPPH, nitric oxide, superoxide, and hydrogen peroxide (H2O2) radicals in a concentration-dependent manner. The content of total phenols, flavonoids, and tannins was also high in the hydroethanolic extract. The antioxidant capacity and total phenolic content were shown to be significantly related, indicating that phenolic chemicals are the primary contributors to plants' antioxidant capabilities. Based on the findings, it can be concluded that the hydroethanolic leaf extract of E. tectorius could serve as a potential source of antioxidants and can be explored as a therapeutic agent in free radical-induced diseases.

Myrciaria floribunda (H. West ex Willd.) O. Berg, Myrtaceae, is a native plant species of the Atlantic Rain Forest, from north to south of Brazil. The lyophilized ethyl acetate extract from the leaves of M. floribunda was investigated for its antiproliferative activity in tumor cell lines, antioxidant capacity and its total phenolic, flavonoid and tannin contents. Antiproliferative activity was tested in vitro against seven human cancer cells and against immortalized human skin keratinocytes line (HaCat, no cancer cell). Antioxidant activity was determined using 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and oxygen radical absorbing capacity (ORAC) assays and total phenolic, flavonoid and tannin contents were determined by spectrophotometric techniques. Ethyl acetate extract of M. floribunda exhibited antiproliferative activity against cancer cell lines with total growth inhibition (TGI) between 69.70 and 172.10 µg/mL. For HaCat cell, TGI value was 213.60 µg/mL. M. floribunda showed a strong antioxidant potential: EC50 of 45.89±0.42 µg/mL and 0.55±0.05 mmol TE/g for DPPH and ORAC, respectively. Total phenolic content was 0.23±0.013g gallic acid equivalents (GAE)/g extract and exhibited 13.10±1.60% of tannins content. The content of flavonoid was 24.08±0.44% expressed as rutin equivalents. These results provide a direction for further researches about the antitumoral potential of M. floribunda.

Objective: The aim of this study is to evaluate the antioxidant potential, determination of total phenolic and flavonoid content in nine selected medicinal plants Spondias pinnata, Melia azedarach, Ageratina adenophora, Urtica dioica, Curcuma longa, Bauhinia variegata, Elaeocarpus angustifolius Blume, Achyranthes aspera, and Psidium guajava from Kavre district of Nepal using in vitro studies.
 Methods: Methanolic plant extracts were prepared by cold percolation method. The methanol extract of nine medicinal plants collected from Kavre district of Nepal, was screened for assessing bioactive phytoconstituents followed by antioxidant property, total phenolic, and flavonoid content. Different plants collected were powdered and extracted with methanol, concentrated by a rotatory evaporator and analyzed for the presence of phytochemicals. The antioxidant potential of the plant extracts was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay.
 Results: The phytochemical analysis of methanolic extracts of all nine medicinal plants displayed the presence of various secondary metabolites such as alkaloids, flavonoids, polyphenols, saponins, and quinones. The extract of S. pinnata showed the highest percentage of radical scavenging activity up to 87.94±1.88 with 50% inhibitory concentration (IC50) 17.51±1.27 μg/mL, followed by B. variegata, 80.63±1.06 with IC50 value 26.55±2.61 μg/mL. The standard, ascorbic acid has IC50 value of 20.13±1.17 μg/mL. Further, the ethyl acetate fraction of S. pinnata showed the maximum percentage of radical scavenging (85.92±1.37) with IC50 value of 46.95±1.17 μg/mL. Moreover, S. pinnata displayed the highest total phenolic content (TPC) 48.26±1.23 mg GAE/g (milligram gallic acid equivalent per gram) extract while the highest flavonoid content was displayed by Melia azedarach 41.07±1.53 mg QE/g (milligram quercetin equivalent per gram) extract measured by the Folin–Ciocalteu reagent method and aluminum chloride colorimetric method.
 Conclusions: The preliminary results of this study have put forward the extract of S. pinnata showed the highest percentage of radical scavenging activity and S. pinnata displayed the highest TPC while the highest flavonoid content was displayed by Melia azedarach methanolic extracts although the further studies are needed to assess its mechanism of action.

In this study, the antioxidative activity and polyphenolic content of Syzygium aromaticum's flower bud were compared under different extraction solvents including chloroform, ethyl acetate, methanol, and aqueous. The antioxidant activity was assessed via established in vitro assay models such as 2, 2-diphenyl-1-Picrylhydrazyl (DPPH) radical scavenging assay, NO− radical scavenging assay, H2O2 scavenging assay and Fe3+ reducing capacity. Total phenolic content was measured according to Folin–Ciocalteu's method, and total flavonoid content was estimated by using the aluminum chloride colorimetric method. The results showed that aqueous extract possessed the highest TPC (19.11 ± 2.76 mg GAE/g DW) and TFC (15.32 ± 1.53 mg CtE/g DW). Among the extracts, methanol extract exerted the strongest radical DPPH quenching activity with an IC50 value of 303.56 ± 13.14 μg/mL. The highest NO− radical scavenging activity was shown by methanol extract (IC50192.94 ± 1.9 μg/mL) which is stronger than BHT (IC50247.64 ± 12.89 μg/mL). Methanol extract showed a strong H2O2 scavenging activity (IC50233.71 ± 3.72 μg/mL). The highest Fe3+ reducing capacity was shown by methanol extract (Absorbance = 0.36 ± 0.05). Strong and positive correlations were observed between total phenolic and flavonoid contents and the antioxidant assays. The results of the present work revealed that the tested spice demonstrated high antioxidant activity, total phenolics, and flavonoids. Thus, this spice is worth considering as important source of natural antioxidant agents.

Eriobotrya plants are known to have significant amounts of phenolics and flavonoids, and exhibit a strong antioxidant activity. Experiments were conducted to examine variation in the contents of total phenolics and flavonoids, and antioxidant activities in the leaves of 11 Eriobotrya species (Tibet loquat, Daduhe loquat, Hengchun loquat, Taiwan loquat, Oak leaf loquat, Bengal loquat, Fragrant loquat, Guangxi loquat, Obovate loquat, Big flower loquat, and common loquat, the last species include two materials, one is a cultivar 'Zaozhong 6', another is a wild tree). In these species, 'Zaozhong 6' loquat is a cultivar. The leaf extracts of 'Tibet', 'Obovate', 'Taiwan', 'Bengal' and 'Hengchun' loquats exhibited significantly higher contents of total flavonoids and total phenolics, compared with those of other species. Of these 11 species, the highest contents of total phenolics and total flavonoids were observed in 'Tibet' and 'Obovatae' loquats, respectively. The significantly stronger antioxidant abilities assessed by the DPPH radical scavenging activity and reducing power were obtained in the leaf extracts of 'Taiwan', 'Tibet', 'Bengal', 'Oak leaf', 'Hengchun' and 'Obovate' loquats, compared with the other species. In addition, significant correlations were found between the contents of total phenolics or flavonoids and DPPH radical scavenging activity/reducing power. This work indicates that the leaf extracts of the wild Eriobotrya species, 'Tibet', 'Obovatae', 'Taiwan', 'Bengal', 'Oak leaf' and 'Hengchun' loquats, exhibited significantly higher levels of total phenolics and flavonoids, and significantly stronger antioxidant activities, compared with the cultivated species, 'Zaozhong 6' loquat, which suggests that these wild species have a better utilization value.

The purpose of the current study was to investigate total phenolic (TPC), total flavonoids (TF) and flavonols (FV) contents, as well as antioxidant and antimicrobial activities of different extracts of <em>Pistacia lentiscus </em>leaves. TP compounds were quantified by Folin-Ciocalteu, while antioxidant properties were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), radical scavenging assay (RSA), Ferric reducing activity power (FRAP) and β-carotene bleaching (βCB) assay. The antimicrobial activity was assessed against Gram-positive bacteria (<em>Micrococcus luteus</em>, <em>Bacillus subtilis </em>and<em> Listeria innocua</em>), Gram-negative bacteriaum (<em>Escherichia coli</em>) and fungi (<em>Candida pelliculosa </em>and <em>Fusarium oxysporum albidinis</em>). Overall, the ethanol fraction (EEPL) exhibited the highest TPC (70.4 ± 7.07 mg GAE g^-1 powder), TF (32.06 ± 1.68 mg QE g^-1 powder), FV (14.36 ± 1.68 mg QE g^-1 powder) and RSA (IC₅₀= 5.34 ± 0.2 µg mL^-1). Whereas, methanol extract (EMPL) displayed the highest FRAP (1.20 ± 0.02) and dichloromethane extract (EDPL) showed a high βCB (90.32 ± 2.98%). Furthermore, the antioxidant activities of extracts exhibited strong positive correlation with TP, TF and flavonols. The extracts obtained showed good inhibitory effect against most of the bacterial and fungal strains tested, suggesting their possible use as antimicrobial agents in medical or agro-industry sectors.

Aims: This study aimed to investigate the total phenolic and flavonoid content and DPPH free-radical scavenging activity of Vernonia amygdalina planted in Mekong Delta. The optimized conditions for maceration of pandan leaves included drying method, ratio of pandan leaf powder-to-solvent, and extraction time.&#x0D; Methodology: The fresh pandan leaves were divided into two equal portions, subjected to different drying methods: shade and oven drying. The dried leaf powder was macerated in ethanol at room temperature. The maceration was conducted with 3 different ratios of pandan leaf powder-to-solvent (w/v) (1:10, 1:15 and 1:20), and the extraction time was 1, 2 and 3 days. The total flavonoid content was determined using aluminum chloride method whereas the total phenolic content was assessed using Folin-Ciocalteu assay. Meanwhile, the antioxidant activity of the plant extracts was quantitatively evaluated using DPPH test.&#x0D; Results: The results indicated that the best conditions for maceration of pandan leaves were 1:10 shade-dried leaf powder-to-solvent ratio in 1-day extraction time. Accordingly, the total flavonoid and phenolic content was found to be the highest value of 130.02 ± 2.24 mg QE/g of dried extract and 100.67 ± 1.76 mg GAE/g of dried extract (p &lt; 0.05), respectively. The lowest IC50 of DPPH free-radical scavenging activity of pandan leaf extract was found to be 0.90 ± 0.02 mg/mL (p &lt; 0.05). In addition, the Pearson’s correlation coefficient between IC50 of DPPH free-radical scavenge activity and total flavonoid content was R2 = 0.74 compared to that of phenolic content with the value of R2 = 0.69, indicating that the IC50 of DPPH free-radical scavenge capacity of pandan leaves was influenced chiefly by flavonoid compounds.&#x0D; Conclusion: There was a significant difference in phenolic and flavonoid content and DPPH free-radical scavenging activity between shade-dried and oven-dried pandan leaf extracts.

Syzygium cumini is commonly used in India as a traditional medicine. In this study, the antioxidant and antimicrobial potentials of different solvent extracts of S. cumini leaves were investigated. Extraction was done sequentially in Soxhlet apparatus, using various solvents (petroleum ether, toluene, ethyl acetate, acetone and water). Antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay, hydroxyl radical scavenging assay, superoxide anion radical scavenging assay and reducing capacity assessment. Total phenol and flavonoid content was also measured. The antimicrobial activity was done by agar well diffusion method against some of the tested foodborne, pathogenic and skin disease causing microorganisms. The acetone extract had more total phenol content and more antioxidant activities, conforming to the hypothesis that phenol content and antioxidant activity has a direct correlation. The acetone extract had notable antimicrobial effect on microorganisms. The results obtained appeared to confirm the antioxidant and antimicrobial potentials of the S. cumini. PRACTICAL APPLICATIONS Food safety is a fundamental concern of both consumers and the food industry, especially as the number of reported cases of food-associated infections continues to increase. It has been estimated that as many as 30% of people in industrialized countries suffer from foodborne diseases each year. Microorganisms play a major role in contamination of stored foods deteriorating them quantitatively as well as qualitatively. Fungi are significant destroyer of foodstuffs during storage, rendering them unfit for human consumption by retarding their nutritive value and sometimes by producing mycotoxins. In the present study, antioxidant and antimicrobial activities of different solvent extracts of Syzygium cumini leaves was evaluated. Acetone extract had good antioxidant activity comparable with standard and remarkable antimicrobial activity. The results indicate that S. cumini may become important natural source of compounds with health protective potential and antimicrobial agent, which can be used in pharmaceutical, nutraceutical and food preparation.

Phenolic and flavonoid contents of mandarin (Citrus reticulata Blanco) fruit tissues and their antioxidant capacity as evaluated by DPPH and ABTS methods

BackgroundBee products are gaining interest in the field of research due to their biochemical and nutritive properties. Honey bee products have been researched extensively but little has been done in regards to stingless bees. There are many species of stingless bees including Plebeina armata, which are found in the Afrotropics. They are underground nesting and produce honey, propolis, wax, pollen and bee bread. These products are known to be rich in polyphenols that comprise of flavonoids and phenolic. In our study, we analysed colorimetrically the total flavonoid, phenolic content and radical scavenging activity of honey (n = 22) and propolis (n = 25) from Bomet, Kisii and Maralal in Kenya.ResultsHoney and propolis had total flavonoid content of 12.00–22.67 mg QE/100 g and 288.15–944.76 mg QE/100 g while total phenolic content was 87.01–239.93 mg GAE/100 g and 524.14–1225.01 mg GAE/100 g, respectively. In considerations to the regions, Maralal had the highest phenolic and flavanoid content followed by Bomet and Kisii was the least. The same trend was observed in the radical scavenging activity. Except for the total flavonoid content in honey, the difference was significant (p &lt; 0.05).ConclusionThe polyphenol content of both honey and propolis of P. armata are equally affected by geographical location as a result of different vegetation. They are good source of antioxidants, which can be utilized in diet due to their radical scavenging properties.

This study presents the effect of hydrolysis on the antioxidant activity of olive mill waste. The olive pomace samples were collected at different stages of maturity and were investigated for their phenolic content and antioxidant activity. Three different extraction procedures were employed, including methanolic maceration extraction and two hydrolysed procedures using 6 M HCL for acid hydrolysis and 10 M NaOH for alkaline hydrolysis. The total phenolic, flavonoid and ortho-diphenolic content, metal ion reducing activity, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and 2,2-Diphenyl-1-picrylhydrazyl Radical Scavenging, hydrogen peroxide and superoxide scavenging activity assays were determined for the different extracts. In this study, cultivar and maturation of olives was one of the factors that affected the phenolic content in the olive pomace samples. Results show that alkaline hydrolysis had the highest antioxidant activity with respect to total phenolic content, 2,2-Diphenyl-1-picrylhydrazyl scavenging activity, metal ion reducing activity and superoxide scavenging activity, whereas acid hydrolysis had the highest 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) scavenging activity. The correlation analysis carried out on the different phenolic classes revealed that the total phenolic, flavonoid and ortho-diphenolic content were correlated with metal ion reducing activity and Radical Scavenging activity.