PHYTOCHEMICAL CONTENT AND ANTIOXIDANT ACTIVITIES IN DIFFERENT ORGANS OF POMELO (CITRUS MAXIMA [BURM.] MERR.) USING 2,2-DIPHENYL-1-PICRYLHYDRAZYL AND PHOSPHOMOLYBDENUM ASSAYS

Abstract

<p>ABSTRACT<br />Objectives: The aims of this research were to determine antioxidant activity from various organs extracts of pomelo using 2,2-diphenyl-1picrylhydrazyl<br />(DPPH)<br />and<br />phosphomolybdenum<br />assays,<br />total<br />phenolic,<br />flavonoid,<br />and<br />carotenoid<br />content,<br />correlation<br />of<br />total<br />phenolic,<br />flavonoid,</p><p>and<br />carotenoid<br />content<br />in<br />various<br />extracts<br />of<br />chayote<br />with their<br />inhibitory<br />concentration<br />50%<br />(IC<br />) of DPPH antioxidant activities and exhibitory<br />concentration 50% (EC<br />50<br />) of phosphomolybdenum capacity, and correlation between two antioxidant assays.<br />50<br />Methods: Extraction was carried out by reflux using various polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant<br />activities using DPPH and phosphomolybdenum assays, determination of total phenolic, flavonoid, and carotenoid content were conducted by UVvisible<br />spectrophotometry<br />and its correlation<br />with IC<br />Results: The lowest IC<br />50<br />50<br /> of DPPH and EC<br />50<br /> of phosphomolybdenum was analyzed by Pearson’s method.<br /> of DPPH scavenging activity was shown by ethyl acetate cortex extract of pomelo (0.68 µg/ml), whereas the lowest EC<br /> of<br />phosphomolybdenum capacity was given by ethyl acetate leaves extract of pomelo (101.36 µg/ml). Ethyl acetate cortex extract of pomelo had the<br />highest total phenolic content and ethyl acetate leaves extract had the highest total flavonoid content (TFC). There was a negative and significant<br />correlation between TFC in cortex and peel extracts of pomelo with their IC<br />50<br /> of DPPH.<br />Conclusions: All organs extracts of pomelo (except n-hexane peel extract) were classified as a very strong antioxidant by DPPH method. Flavonoid<br />compounds in cortex and peel extract of pomelo were the major contributor in antioxidant activities by DPPH method. DPPH and phosphomolybdenum<br />assays gave no linear results in antioxidant activities of leaves, cortex, and peel extracts of pomelo.<br />Keywords: Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Phosphomolybdenum, Pomelo, Three organs.<br />50</p>