Physiological significance of the binding of acidic isoperoxidases to cell walls of lupin

Abstract

Intercellular acidic isoperoxidases (EC 1.11.1.7) isolated from exponentially growing lupin (Lupinus albus. L. cv. multolupa) hypocotyls are under the control of exogenously applied auxins. Application of auxins leads to a short‐term reduction in the level of free intercellular peroxidases, and this effect is associated with a binding of these free peroxidases to the cell walls, probably mediated by an acidification of the cell wall. The ratio of free intercellular peroxidases to the total intercellular peroxidase activity, varies along the axis of exponentially growing hypocotyls. It has a V‐shaped distribution with the minimum value in the elongation III‐zone, where high levels of auxins have previously been implied in differentiation. This minimum value coincides spatially with the first signs of cell wall thickening in the hypocotyl cells and, paradoxically, it is out of phase with respect to the maximal cell elongation. On the other hand, the ratio of free intercellular peroxidases reaches its maximal values in both the most undiffercntiated phloem cells and the differentiated xylem cells. High levels of free intercellular peroxidase activity in phloem cells are hard to explain, since phloem cell walls remain unlignified during almost all stages of differentiation. However, association of free intercellular peroxidase activity with xylem cells is clearly associated with the lignification of the xylem cell walls. The physiological significance of the binding vs release of intercellular peroxidase is discussed in relation to the catalytic properties and stability at acidic pH of both the bound and free forms of this enzyme.