Physiological implication of intracellular trehalose and mannitol changes in response of entomopathogenic fungus Beauveria bassiana to thermal stress

Abstract

To explore possible role of intracellular trehalose accumulation in fungal tolerance to summer-like thermal stress, 3-day colonies of Beauveria bassiana grown on a glucose-free medium at 25 degrees C were separately exposed to 35, 37.5 and 40 degrees C for 1-18 h, respectively. Trehalose accumulation in stressed mycelia increased from initial 4.2 to 88.3, 74.7 and 65.5 mg g(-1) biomass after 6-h stress at 35, 37.5 and 40 degrees C, respectively, while intracellular mannitol level generally declined with higher temperatures and longer stress time. The stress-enhanced trehalose level was significantly correlated to decreased trehalase activity (r(2) = 0.73) and mannitol content (r(2) = 0.38), which was inversely correlated to the activity of mannitol dehydrogenase (r(2) = 0.41) or mannitol 1-phosphate dehydrogenase (r(2) = 0.30) under the stresses. All stressed cultures were successfully recovered at 25 degrees C but their vigor depended on stressful temperature, time length and the interaction of both (r (2) = 0.98). The highest level of 6-h trehalose accumulation at 35 degrees C was found enhancing the tolerance of the stressed cultures to the greater stress of 48 degrees C. The results suggest that the trehalose accumulation result partially from metabolized mannitol and contribute to the fungal thermotolerance. Trehalase also contributed to the thermotolerance by hydrolyzing accumulated trehalose under the conditions of thermal stress and recovery.