Physiological and growth responses of two different salt-sensitive cucumber cultivars to NaCl stress

Abstract

The effect of NaCl stress on the growth, membrane permeability, anti-oxidant enzyme activities and ion content of cucumber seedlings was investigated. Two cultivars (Jinchun No. 2, a relatively salt-sensitive cultivar, and Zaoduojia, a relatively salt-tolerant cultivar) of cucumber were used. Shoot and root dry weights, plant height, stem diameter, leaf area and leaf number of both cultivars decreased when NaCl concentrations increased. The decreases in shoot and root dry weights and leaf area were more significant in Jinchun No. 2 than in Zaoduojia. Meanwhile, the salt injury index, the membrane permeability, malondialdehyde (MDA) contents, superoxide dismutase (SOD) and peroxidase (POD) activities of both cultivars increased significantly with salt stress, and the increases in the salt injury index and MDA were higher in Jinchun No. 2 than in Zaoduojia, whereas the increase in POD activity was lower in Jinchun No. 2 than in Zaoduojia. Free proline content of Zaoduojia increased markedly with increasing concentrations of NaCl, whereas the content of Jinchun No. 2 was unaffected by salt stress. In addition, the contents of Na+ in the leaf, stem and root of both cultivars increased significantly, whereas the contents of K+ decreased significantly, resulting in an increase in the Na+/K+ ratio when NaCl concentrations increased. These results suggest that Zaoduojia exhibits a better protection mechanism against oxidative damage and lipid peroxidation by maintaining higher proline content and POD activity than the salt-sensitive Jinchun No. 2 cultivar.