Abstract
Twenty accessions of Vernonia galamensis (Cass.) Less. from six subspecies were studied to develop a phylogram based on the seed protein profiles on one-dimensional (SDS-PAGE) gel electrophoresis. Soluble protein was extracted with 50 mM Tris-HCl buffer. Resolution factor values (Rf) and molecular weights of different protein profiles were estimated. The percentage similarities among different accessions were calculated and a consensus phylogram was derived using PAUP analysis. Almost all soluble proteins showed a moderate mobility (Rf. 0.2 to 0.6) and resolved as four clusters with varying number of classes. Variety ethiopica was observed to have greater mutational differences from other varieties of same subspecies galamensis and other subspecies. Variety petitiana showed greater genetic deviation to varieties galamensis, australis and nairobensis of the same subspecies galamensis than among the later three. Mutational distances between Subspecies gibbosa, mutomoensis and afromontana were similar.
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