Abstract
Twenty-seven Tritirachium strains were present in the URM culture collection originally founded in Brazil by Augusto Chaves Batista. Fifteen freshly-prepared cultures were obtained from these original strains preserved under mineral oil. DNA was extracted for analysing phylogenetic relationships using the sequence information available from Tritirachium type materials and reference strains. Phylogenetic analysis using the internal transcribed spacer (ITS) ribosomal DNA sequences revealed that eight of these strains belong to the same clade as of T. oryzae and that the four other strains belong to the same clade as of T. candoliense. The strain URM 38, which was previously identified as “T. brumptii”, appeared to exist as a single lineage, related to T. roseum and T. candoliense. Based on morphological features and multi-locus phylogenetic analysis, including the analyses of ITS and LSU rDNA, and rpb2 sequences, we propose that URM 38 belongs to the new species T. batistae. This novel species exhibited velutinous to cottony colonies of varying colour, septate hyphae without clamp connections, conidiophores reduced to conidiogenous cells, conidiogenous cells with a distinct sympodial rachis, and single-cell conidia that was globose to subglobose, obovoid, smooth, and hyaline. The morphological features of species accepted in Tritirachium are included in this study.
Highlights
The genus Tritirachium was described by Limber in 1940 to accommodate the fungi with ‘rachis-like or zigzag form of the fertile portions of the erect conidiophore’ (Beguin et al 2012)
Several studies investigated the morphology of these fungi in more details (Limber 1940; Macleod 1954; Hoog 1972; 1973), and more recently, phylogenetic analyses based on DNA sequences were conducted, which placed these fungi in the phylum Basidiomycota (Schell et al 2011; Beguin et al 2012; Manohar et al 2014)
We aimed to revisit the Tritirachium strains that were previously deposited at the URM culture collection, to determine their phylogenetic placement using the internal transcribed spacer (ITS) rDNA sequences of these available strains, to report species isolated from new geographical and substrate/host records, and to provide detailed description of a new species based on the multi-gene phylogenetic analyses (ITS, large subunit (LSU), and rpb2), and its morphological features
Summary
The genus Tritirachium was described by Limber in 1940 to accommodate the fungi with ‘rachis-like or zigzag form of the fertile portions of the erect conidiophore’ (Beguin et al 2012). Manohar et al (2014) described the species T. candoliense, which was isolated from a coastal sediment sample of the Arabian Sea. A similar genus, Paratritirachium, was introduced in the family Tritirachiaceae, which currently accommodates two species, P. cylindroconium and P. curvibasidium (Beguin et al 2012; Nguyen et al 2014; He et al 2019)
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