Abstract
BackgroundAnthrax is a zoonotic disease caused by the gram-positive bacterium Bacillus anthracis. The most anthrax-endemic regions of Russia are Siberia and North Caucasus. Previously, genotyping of Russian B.anthracis isolates was carried out using canSNP and MLVA data; these methods yield lower resolution results compared to whole genome SNP analysis (wgSNP). In this research, we have used wgSNP method for genotyping of 10 B.anthracis isolates, obtained during 1961–2016 in Russia on territory of Western Siberia.ResultsWe have analyzed 185 B.anthracis genomes available in GenBank database and genomes of 10 isolates obtained in this study to determine the place of Russian isolates in the global phylogeny of B.anthracis. For the studied genomes we have detected 7203 SNPs, which were used for building a phylogenetic reconstruction with Maximum Likelihood Method. Results of the phylogenetic analysis indicate that Russian strains belong to three different genetic groups. Three strains belong to genetic group “Ames”, two strains – to “STI” group. Five strains belong to the main genetic line B, and four of them form a subcluster, described for the first time, which we have named “Siberia”.ConclusionsIn this study, the data on genetic diversity of B.anthracis strains on the territory of Western Siberia is presented for the first time. As a result of complex phylogenetic analysis, the place of these isolates was determined in the global phylogenetic structure of the B.anthracis population. We describe a new cluster in the main genetic line B for the first time.
Highlights
Anthrax is a zoonotic disease caused by the gram-positive bacterium Bacillus anthracis
In this study we describe genetic diversity of B.anthracis strains isolated from different sources during anthrax outbreaks in Western Siberia from 1961 to 2016
General results For the research we have selected 10 isolates of B.anthracis obtained on the territory of Western Siberia during anthrax outbreaks registered from 1961 to 2016
Summary
Anthrax is a zoonotic disease caused by the gram-positive bacterium Bacillus anthracis. The most anthrax-endemic regions of Russia are Siberia and North Caucasus. Genotyping of Russian B.anthracis isolates was carried out using canSNP and MLVA data; these methods yield lower resolution results compared to whole genome SNP analysis (wgSNP). We have used wgSNP method for genotyping of 10 B.anthracis isolates, obtained during 1961–2016 in Russia on territory of Western Siberia. Anthrax is a dangerous zoonotic disease caused by an aerobic gram-positive spore-forming microorganism Bacillus anthracis. In their research Hanczaruk M. et al describe another form, injectional, caused by injection of heroin, contaminated with anthrax spores [2]. In Russia, the greatest number of cases are registered on the territory of Siberia and the North Caucasus [5]
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