Abstract
Present study aims at exploring parallel nutrient removal from secondarily treated wastewater and biogas production potential of Chlorella vulgaris. The observed growth rate (per day) and doubling time (days) of C. vulgaris was 0.36 and 0.44, respectively. C. vulgaris has removed 31 % COD, 40 % PO4-P , 36 % NH4-N and 38 % TKN from secondarily treated wastewater using open raceway pond. The biomass was analyzed for proteins, carbohydrates, Lipids, fibres, TS and VS. The optimum loading rate for maximum biogas yield was found to be 2 g VS/L. Effect of various pre-treatment methods (thermal, chemical, sonication and thermo-chemical) has also been studied. The biomass and biomass extract (before and after pre-treatments) were also analyzed for solubilization of complex compounds. Thermally pre-treated biomass has increased biogas production by 60 % (480 mL/g VS) relative to untreated biomass (350 mL/g VS). This study has successfully demonstrated that microalgal cultivation in wastewater can be easily adopted in currently available wastewater treatment plants without any major modifications of existing available infrastructure.
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