Photothermal-Reagent-Triggered Visual Thermoresponsive and Quantized Photoelectrochemical Dual-Signal Assay.

Abstract

In vitro biosensing chips are urgently needed for early-stage diagnosis and real-time surveillance of epidemic diseases. Herein, a versatile zone with photothermal effects is implanted in the miniature space of a collapsible lab-on-paper photoelectrochemical biosensor for on-site detection of microRNA-141 in body fluids, which can flexibly interconnect the traditional photocurrent signal with functional temperature response. The visualized thermoresponsive results are enhanced by the exciton energy conversion between Fe3O4 nanoparticles (Fe3O4 NPs) and formed Prussian blue nanoparticles under near-infrared irradiation, which not only presents heat energy gradient variations but also generates color changes. Significantly, the controlled release of Fe3O4 NPs is actuated by a target-triggered enzyme assist strand displacement cycle strategy to efficiently improve the accuracy of target temperature signal prediction, which can concurrently mediate photoelectric signal attenuation via promoting the rapid recombination of photoexcited charge carriers on the CuInS2/CoIn2S4 electrode surface, affording dependable ultrasensitive detection results. Benefitting from the ingenious design of the versatile thermoresponsive-photoelectric sensing platform, the preliminary screening and ultrasensitive quantitative analysis can be simultaneously achieved in a single-drop sample. As a consequence, speedy prediction results and satisfied monitoring data are acquired in the ranges of 0.5 pM to 2 nM and 0.001 pM to 5 nM by measuring the temperature change and photocurrent intensity. By right of these advantages, such research paves a prospective paradigm for the manufacture of a visual, rapid, broad-spectrum, and reliable real-time surveillance platform, which allows it to be a promising candidate for epidemic disease home diagnosis and intelligent diagnosis.