Abstract
Spinach chloroplasts, isolated rapidly in isotonic media will reproducibly give photosynthetic control rates (State 3/State 4) of 4–6, and ADP/O ratios (equivalent to ATP/2e −) of 1.4 – 2.1 when assayed in slightly hypotonic media. Photosynthetic control can be followed as oxygen evolution with either ferricyanide or NADP as electron acceptors, or as oxygen uptake in the presence of azide, which blocks chloroplast catalase, either alone (endogenous catalyst) or with added methyl viologen. This control can be triggered either by added ADP or by added Pi in all cases. Optimum concentrations of Mg, Pi and EDTA are required; the pH is also critical. Excess EDTA results in an inhibition of electron transport on addition of ADP.
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