Abstract
Objective: The aim of this study was to evaluate the subcutaneous tissue response after different protocols to photodynamic therapy (PDT). In Phase 1, were tested the diode laser (used for 1min) associated to the photosensitizer phenothiazine chloride solution (PCS) in different concentrations. In Phase 2 – the diode laser and LED were tested associated to two different photosensitizers, PCS and Curcumin, in different exposure times of light application. Material and Methods: After 7, 21 and 63-days the animals were euthanized and the subcutaneous tissue processed to histological analysis. Qualitative and semi-quantitative descriptions of the inflammatory process and immunohistochemical technique were performed. The obtained data were analyzed by Kruskal-Wallis and Dunn’s post-test (alpha = 0.5). Results: On Phase 1, the tissue response was very similar among the groups. For the inflammatory infiltrate, PCS with concentration of 10mg/mL exhibited the most intense reaction (p > 0.05). On Phase 2, at 7-days period, the analyzed parameters presented small magnitude and after 21 and 63-days, all the parameters demonstrated tissue compatibility. Conclusion: Both photosensitizers presented proper tissue compatibility regardless the different concentrations used on Phase 1 and different durations of light exposure on Phase 2. Keywords Photodynamic therapy; Phenothiazine chloride solution; Curcumin; Isogenic mice; Subcutaneous tissue.
Highlights
INTRODUCTIONThe photodynamic therapy (PDT) is a treatment in which one photoactive agent, known as photosensitizer, is activated by a light with a specific wavelength, triggering the production of singlet oxygen, superoxide and free radicals (reactive oxygen species), that are cytotoxic to target cells [1,2,3,4] leading to cellular death by oxidation of biological molecules such as proteins, nucleic acids and lipids [5]
The photodynamic therapy (PDT) is a treatment in which one photoactive agent, known as photosensitizer, is activated by a light with a specific wavelength, triggering the production of singlet oxygen, superoxide and free radicals, that are cytotoxic to target cells [1,2,3,4] leading to cellular death by oxidation of biological molecules such as proteins, nucleic acids and lipids [5].The antimicrobial efficiency of PDT in Dentistry has been evaluated in several studies [1,3,4]
Garcia et al, 2014 [8] observed a small and isolated number of inflammatory cells located in dense conjunctive tissue, composed by a large amount of collagen fibers and few fibroblasts, in teeth with induced periodontitis treated by scaling and root planning followed by PDT
Summary
The photodynamic therapy (PDT) is a treatment in which one photoactive agent, known as photosensitizer, is activated by a light with a specific wavelength, triggering the production of singlet oxygen, superoxide and free radicals (reactive oxygen species), that are cytotoxic to target cells [1,2,3,4] leading to cellular death by oxidation of biological molecules such as proteins, nucleic acids and lipids [5]. Garcia et al, 2014 [8] observed a small and isolated number of inflammatory cells located in dense conjunctive tissue, composed by a large amount of collagen fibers and few fibroblasts, in teeth with induced periodontitis treated by scaling and root planning followed by PDT. The results of those three papers showed the importance of the evaluation of different parameters for better results after the use of PDT, without damages for tissue repair process. This study aimed to evaluate the tissue response in mice’s dorsal subcutaneous, after performing PDT, in different conditions: Phase 1 - photosensitizer phenothiazine chloride solution in different concentrations and Phase 2 - two photosensitizers (Phenothiazine chloride solution and Curcumin), with the different laser exposure times
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
