Abstract
A photo-responsive membrane was prepared by casting a solution of 11- cis-retinal, phosphatidyl choline, and triacetyl cellulose. The membrane-bound 11- cis-retinal exhibited three steps of photospectroscopic change on light irradiation. The photoisomerization of the membrane-bound 11- cis-retinal appeared to be complete on light irradiation for 1-2 rain. The potential difference across the membrane drastically changed under these conditions. On the other hand, a membrane incorporating only 11- cis-retinal exhibited little light-induced transmembrane potential. The light-induced change in membrane potential occurred in association with the photo-isomerization of the membrane-bound 11- cis-retinal. Phosphatidyl choline is thought to play an important role in transducing the conformational change of 11- cis-retinal to the dynamic photo-response in membrane potential.
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