Abstract
The fluorescence emission of merocyanine 540 (MC540) in 95% ethanol was studied under continuous irradiation. Fluorescence spectra from excited states of both normal (N) and photoisomeric (P) species are identical. The laser fluence dependence of the fluorescence intensity is interpreted on the basis of a photochromic system involving N and P ground states and first excited singlet states. Common flash photolysis equations are generalized in order to include a photoequilibrium between isomers. The emission data are used together with previous flash photolysis and optoacoustic results to obtain P fluorescence and photoisomerization quantum yields as 0.07 {plus minus} 0.02 and 0.20 {plus minus} 0.04, respectively, P absorption cross section at the maximum (560 nm) as 4.74 {times} 10{sup {minus}16} cm{sup 2} (125 {times} 10{sup 3} M{sup {minus}1}{center dot}cm{sup {minus}1}), and the energy difference between the ground states as 165 kJ{center dot}mol{sup {minus}1}.
Highlights
Merocyanine 540 (MC540) is an anionic polymethine dye with various applications in photobiology.1 Since 1974 it has beenMC 540 used as a probe to monitor the state of membrane polarization through changes in its absorption or fluorescence properties.2 It was later employed to selectively stain and kill leukemic and immature hemopoietic cells upon irradiation.3 its ability to sensitize the inactivation of encapsulated viruses was reported.4 In view of the multiple applications to photobiology, many studies were devoted to the interaction of merocyanine 540 (MC540) with micelles,5 liposomes,6 and membranes.7(1) For a recent review see: Sieber, F
The fluorescence emission of merocyanine 540 (MC540) in 95% ethanol was studied under continuous irradiation
Common flash photolysis equations are generalized in order to include a photoequilibrium between isomers
Summary
Merocyanine 540 (MC540) is an anionic polymethine dye with various applications in photobiology. Since 1974 it has beenMC 540 used as a probe to monitor the state of membrane polarization through changes in its absorption or fluorescence properties. It was later employed to selectively stain and kill leukemic and immature hemopoietic cells upon irradiation. its ability to sensitize the inactivation of encapsulated viruses was reported. In view of the multiple applications to photobiology, many studies were devoted to the interaction of MC540 with micelles, liposomes, and membranes.7(1) For a recent review see: Sieber, F. Merocyanine 540 (MC540) is an anionic polymethine dye with various applications in photobiology.. MC 540 used as a probe to monitor the state of membrane polarization through changes in its absorption or fluorescence properties.. It was later employed to selectively stain and kill leukemic and immature hemopoietic cells upon irradiation.. Its ability to sensitize the inactivation of encapsulated viruses was reported.. In view of the multiple applications to photobiology, many studies were devoted to the interaction of MC540 with micelles, liposomes, and membranes.. (1) For a recent review see: Sieber, F.
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