Abstract

A photoacoustic spectrum of a smear of whole blood clearly shows all of the details of the haem absorption in an optical spectrum and good optical absorption data without a data extraction procedure. There are three peaks; γ (420 nm), β (550 nm), and α (585 nm) in the band 350–700 nm. For iron-deficiency anaemia the peak/peak ratios, ratios of the value of peak γ to that of the peak β and peak α, and the integral/integral ratios, ratios of the value of the integral under the peak γ to that under the peak β, peak α, peak β and peak α are closely related to the variation of haemoglobin concentration. Linear relationships among the ratios and the Hb concentration are found. These ratios could be a good index of haem synthesis disturbance.

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