Abstract
Photoacoustic spectroscopy is introduced as a promising experimental technique to investigate fungus infected Acai (Euterpe oleracea) seeds. Photoacoustic spectra of healthy and infected Acai seeds with the fungus Colletotrichum gloeosporioides were recorded firstly in the modulation frequency range of 5Hz to 700 Hz, while keeping the wavelength of excitation radiation of a Xe arc-lamp constant, to ascertain the depth of penetration of infection within the seed and secondly, at variable wavelength (wavelength scanning) in the interval 250nm to 1,000 nm, while keeping the modulation frequency constant. In the former, the photoacoustic signal strength from the infected seed was found higher than that of the healthy one, and has been associated with the appearance of new biomolecules associated with the pathogen infection. In the latter, characteristics peaks and bands were observed in the range from 650 nm to 900 nm ascribed to organic compounds with carboxylates and amines (functional groups) forming the typical metabolic structures of the fungus.
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