Abstract
A highly sensitive phosphorimetric method for the assay of β-glucuronidase in biological samples is described. p-Nitrophenol, formed enzymatically from p-nitrophenyl β-D-glucuronide, is extracted with ether and determined phosphorimetrically in a mixture of ether and ethanolic potassium hydroxide. The method is rapid, precise, and very sensitive, requiring as little as 0.5–5 μl of human serum or urine, or 0.3–3.0 μg of protein of rat tissue. The limit of detection for the p-nitrophenol formed is 20 pmol.
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