Abstract
Phosphate was shown to reverse the in vitro activity of phosphonate ions against P. capsici (strain 375) more efficiently in liquid than in solid media. Phosphonate transport by mycelia incubated in aqueous solutions was enhanced by a previous phosphate starvation and the presence of K+ cations. The intracellular phosphonate concentration reached a constant level and this concentration, which is a function of the external concentration, fits a hyperbolic relationship. Phosphonate transport was greatly stimulated when mycelia were incubated in modified Ribeiro's medium. In the absence of any phosphorus source in the growth medium, the phosphate content of mycelia at stationary phase of growth decreased. This sign of phosphate deficiency was intensified in the presence of phosphonate.
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