Abstract
Phospholipase B activity was detected in liquid and agar cultures of Phytophthora infestans and in infected potato leaves. The phospholipase activity in culture filtrates was stimulated 15-fold by deleting glucose from liquid rye medium. An additional 35-fold stimulation occurred after adding PC to the cultures. Much less phospholipase activity was found when the fungus was grown on liquid V/8 medium, and no phospholipase activity was detected with liquid pea, bean or synthetic media, even after the addition of PC. The PC-stimulated rye culture filtrates exhibited not only phospholipase activity, but also galactolipase and acylglycerol lipase activities. The apparent K m for PC (dipalmitoyl) was 2·86 μ m. The phospholipase activity from fungal cultures exhibited optimal activity at pH 9·0 and was strongly inhibited by DTT. During the infection of potato leaves there was a large (more than 14-fold) increase in phospholipase activity. The phospholipase activity in infected leaves had a major peak of activity at pH 9·0, which was inhibited by DTT and a minor peak of activity at pH 6·0 which was insensitive to DTT.
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