Abstract
Activity of phosphofructokinase (PFK) was measured in testes of rats during sexual maturation to determine whether the enzyme was concentrated in spermatogenic or interstitial elements. PFK was also assayed following incubation at 29-38#{176} C in vitro and following short term cryptorchidism to measure the lability of the enzyme to different temperatures. Specific activity (s.a.) of PFK decreased (P<0.05) from 11.81 ± 0.37 units/mg protein in testes from 20-day-old rats to 7.19 ± 0.18 units/mg at 30 days and 6.00 ± 0.14 units/mg at 40 days of age. Activity leveled off at 6.406.50 units until 60 days, then increased to 8.01 ± 0.43 at 120 days of age. Cryptorchidism in adult rats reduced s.a. of PFK to 93% of controls (P<0.05) in 12 h and to 80% of controls (P<0.05) in 48 h. Incubation for 2 h in vitro resulted in higher (P<0.05) s.a. of PFK in testes incubated at 32#{176}C than at 29, 35 or 38#{176}C, but s.a. of PFK in kidney slices was unaffected by incubation temperature. Liver PFK activity decreased with increasing temperature. The results suggest that testic� ular PFK is more concentrated in interstitial tissues than in spermatogenic elements, but that PFK activity in the latter is reduced by abdominal temperatures.
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