Abstract
Objective To investigate the interaction between colon cancer cells and hepatic cell (L02), and it’s influence to Snail expression in tumor cells. Methods Lo Vo cells which transfected with phosphatase of regenerating liver- 3(PRL- 3)and human hepatic cells(L02)simulated tumor microenvironment were cocultured by 0- 5 day, reverse transcriptase- polymerase chain reaction (RT- PCR)and Western blotting were used to examine Snail expression of Lo Vo cells, and the migration of Lo Vo cells also be detected. Results Used by RT- PCR and Western blotting it was found out that Lo Vo cells(transfected with PRL- 3) were reduced Snail express after cocultured with hepatic cells, the inhibition rates were 34. 06%, 37. 82%, 39. 91%, 64. 07%, 73. 58%(P< 0. 05), LoVo cells(without transfected PRL- 3) decreased expression of Snail was not obvious.Besides, coculture also depress the migration of Lo Vo cells. Conclusion PRL- 3 depress the migration of Lo Vo cells through reduced Snail expression in liver metastasis of colon cancer microenvironment. Key words: Phosphatase of regenerating liver-3; LoVo; Hepatic cell; Snail
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