Abstract
Summary Lactococcus lactis subsp. lactis, Lactococcus garvieae and Lactococcus raffinolactis were identified in samples from different domestic animal species, mainly in tonsils of ruminants, dogs and cats, and milk samples from cases of subclinical mastitis in dairy cows. Species differentiation was mainly based on SDS-PAGE analysis of whole-cell protein patterns. A comparison was made with results of biochemical activity tests and sensitivity patterns to clindamycin and oxacillin. These results were used to construct an improved identification scheme for lactococci from animal origin. By SDS-PAGE analysis of whole-cell proteins, Enterococcus seriolicida was shown to be closely related to Lactococcus garvieae .
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