Abstract
Tobacco leaf tissue was injected with avirulent (Bl) and virulent (K60) strains of Pseudomonas solanacearum . Avirulent bacteria induced a hypersensitive reaction (HR), whereas virulent bacteria caused a susceptible response. Ultracytochemical methods for localization of phenolics and polyphenoloxidase (PPO) over a 30-h period after inoculation showed cytoplasmic phenolic deposition at 10 h followed by PPO at 20 h during the HR. Polyphenol-oxidase was localized primarily in the grana of chloroplasts, but also occurred in the cytoplasm and in vesicles found between the plasmalemma and cell wall. Virulent bacteria caused only small increases in phenolic deposition and little observable PPO during the 30 h following inoculation.
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