Abstract

Rosa banksiae Ait. (R. banksiae) is a traditional Chinese folk medicine and an ornamental plant. Most previous studies have focused on cultivation and utilization while there are few research papers on the pharmacological activity of R. banksiae. This study aimed to get a better understanding of R. banksiae by extracting polyphenols with fractionated extraction technology. The results showed that ethyl acetate phase (EAP) contained the most polyphenols, while water phase (WP) had the least. HPLC analysis indicated that rutin and luteolin-4′-O-glucoside existed in the EAP and butanol phase (BP), but quercetin was only detected in the EAP. Six phenolic compositions were not detected in WB. The antioxidant and anti-tumor abilities of the EAP and BP were excellent. The results revealed that R. banksiae possessed a great antioxidant capacity and was rich in polyphenols, thus indicating R. banksiae was suitable for being a natural antioxidant and an abundant source of polyphenols.

Highlights

  • In the process of human metabolism, a large number of free radicals will be produced

  • We have found that the flowers from R. banksiae are used as a raw material for making pastry, such as moon cakes, and extracts of R. banksiae contained a certain level of polyphenols

  • high performance liquid chromatography (HPLC) was successfully applied to identified phenolic components such as rutin, luteolin-40 -O-glucoside and quercetin in three phases

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Summary

Introduction

In the process of human metabolism, a large number of free radicals will be produced They have strong oxidation, which will cause damage to the function of biofilms and cells in the human body, thereby increasing the risk of cardiovascular disease and cancer, and accelerating the process of human aging [1]. Polyphenols are a kind of secondary metabolites with polyphenol structure which exist widely in plants, and have highly efficient bioactivities They possess strong antioxidant abilities [5], with obvious effects of anti-mutation [6], anti-cancer [7], and inhibiting hypertensive [8]. Polyphenols from betel nut tannins have been used to fight tooth decay, because they bind to the proteins of microbes and viruses, leading to the deactivation of proteins [10] These bioactivities have something to do with inhibition of microbial metabolic enzymes or complexation of trace metal ions by polyphenols

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