Abstract
O. fragrans has slightly less antioxidative activity than green tea. Five phenolic compounds, tyrosyl acetate (1), (+)-phillygenin (2), (8E)-ligustroside (3), rutin (4), and verbascoside (5), were isolated from the CHCl3 sub-extract of O. fragrans. The structures were elucidated by interpreting their spectral data. Evaluation of the antioxidative property of the isolated (+)-phillygenin (2), rutin (4), and verbascoside (5) revealed strong DPPH radical scavenging activity, with IC50 values of 19.1, 10.3, and 6.2 μM, respectively. These isolates also exhibited an H2O2 scavenging ability, with IC50 values of 10.5, 23.4, and 13.4 μM, respectively.
Highlights
In China, some flowers are commonly added to tea to increase or improve its taste
This study describes the isolation, structural elucidation of the purified compounds, and evaluates their antioxidative activity against DPPH radical and hydrogen peroxide (H2O2)
The phenolic compounds were the dominant antioxidants in the methanol extract of O. fragrans
Summary
In China, some flowers are commonly added to tea to increase or improve its taste. The added flowers are Gomphrena globosa, Helichrysum bracteatum, Chrysanthemum morifolium, Momordica grosvenori, Chrysanthemum indicum, Nelumbo nucifer, Osmanthus fragrans, and others. The total phenolic content of O. fragrans (291.3 mg/g extract) was determined to be a little less than that of green tea (325.9 mg/g extract), but it was the highest among the seven flowers tested (Figure 1b). Pale yellow, golden yellow, or orange yellow with a four-lobed corolla, and have a strong fragrance [3] It is used as an ornamental plant, and as an additive in food, tea, and other beverages because of its strong fragrance. The flower of O. fragrans has been demonstrated to exhibit antioxidative activity [6], to inhibit NO production [7], to have a neuroprotective effect [8], and to. This study describes the isolation, structural elucidation of the purified compounds, and evaluates their antioxidative activity against DPPH radical and hydrogen peroxide (H2O2)
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