Abstract

In this study, the phenol degradation capacities of four different mixed cultures, namely, Ankara Stream (AS), petroleum-contaminated soil (PS), olive mill wastewater (OMW), and drug mill wastewater (DMW) were investigated with regards to different pH levels, phenol (497.2–1183.0 mg/L) and biomass concentrations (0.5–3.0%(v/v)). The most efficient culture was selected and the optimal conditions required for its highest performance in phenol degradation were studied. We found 100% phenol degradation for DMW at nearly 1000 mg/L initial concentration. The optimum pH was 8 and the mixed culture could tolerate phenol levels up to approximately 1500 mg/L. The selected culture degraded all of the phenol concentrations ranging from 497.2 to 1183.0 mg/L with 100% efficiency after 48 h and 120 h incubation, respectively. Increasing the biomass concentration from 0.5% to 3.0% (v/v) level decreased the incubation time needed for complete degradation. The data indicate that the mixed culture used in this study can be taken as a good candidate for effective treatment of waters contaminated by phenol.

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