Abstract
This study investigated whether pharmacological postconditioning with lactic acid and hydrogen rich saline can provide benefits similar to that of mechanical postconditioning. To our knowledge, this is the first therapeutic study to investigate the co-administration of lactic acid and hydrogen. SD rats were randomly divided into 6 groups: Sham, R/I, M-Post, Lac, Hyd, and Lac + Hyd. The left coronary artery was occluded for 45 min. Blood was withdrawn from the right atrium to measure pH. The rats were sacrificed at different time points to measure mitochondrial absorbance, infarct size, serum markers and apoptotic index. Rats in Lac + Hyd group had similar blood pH and ROS levels when compared to the M-Post group. Additionally, the infarct area was reduced to the same extent in Lac + Hyd and M-Post groups with a similar trends observed for serum markers of myocardial injury and apoptotic index. Although the level of P-ERK in Lac + Hyd group was lower, P-p38/JNK, TNFα, Caspase-8, mitochondrial absorbance and Cyt-c were all similar in Lac + Hyd and M-Post groups. The Lac and Hyd groups were able to partially mimic this protective role. These data suggested that pharmacological postconditioning with lactic acid and hydrogen rich saline nearly replicates the benefits of mechanical postconditioning.
Highlights
This study investigated whether pharmacological postconditioning with lactic acid and hydrogen rich saline can provide benefits similar to that of mechanical postconditioning
We investigated whether the combination of lactic acid and hydrogen rich saline produces the protective effects of postconditioning in a rat model of acute myocardial infarction
Recordings of the hemodynamic parameters heart rate and mean arterial pressure (MAP) indicated that heart contractility was not affected in the various experimental groups following ischemia and reperfusion (Table 1)
Summary
This study investigated whether pharmacological postconditioning with lactic acid and hydrogen rich saline can provide benefits similar to that of mechanical postconditioning. Rats in Lac 1 Hyd group had similar blood pH and ROS levels when compared to the M-Post group. The infarct area was reduced to the same extent in Lac 1 Hyd and M-Post groups with a similar trends observed for serum markers of myocardial injury and apoptotic index. The Lac and Hyd groups were able to partially mimic this protective role These data suggested that pharmacological postconditioning with lactic acid and hydrogen rich saline nearly replicates the benefits of mechanical postconditioning. We investigated whether the combination of lactic acid and hydrogen rich saline produces the protective effects of postconditioning in a rat model of acute myocardial infarction. Lactic acid alone had no significant effect when compared to the R/I group (P . 0.05)
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