Abstract
In normal hairless mouse epidermis the cellular respiration (QO2) on endogenous substrate in vitro is more sensitive to pH variations than the respiration with glucose in the medium. During the first week after painting with the strong carcinogen 20-methylcholanthrene (MCA) the QO2 of hairless mouse epidermis is not more susceptible to pH variations than that of untreated epidermis. A transient broader pH optimum of the respiration to the acidic side after application of the solvent benzene or the irritant cantharidin in benzene is probably due to intracellular accumulation of lactic acid. While the tetrazolium method shows a specific reaction pattern after MCA painting, the oxygen consumption mostly shows an unspecific pattern, reflecting altered cell population kinetics in the epidermis. After MCA treatment there is a discrepancy between oxygen consumption and formazan deposition until the 7th day. The oxygen consumption is decreased while the tetrazolium method gives increased or normal values. A possible explanation is that the mitochondrial membrane has an increased permeability for tetrazolium.
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