Peroxidase cytochemistry and ultrastructure of resident macrophages in fetal rat liver: A developmental study

Abstract

The peroxidase cytochemistry and the ultrastructural characteristics of resident macrophages in fetal rat liver have been investigated. Livers of 10-, 11-, 14-, 17-, and 20-day-old fetuses were fixed by immersion or perfusion, incubated for peroxidase, and processed for transmission electron microscopy. Some 17- and 20-day-old fetuses were injected prior to sacrifice with carbon or 0.8-μm latex particles through the umbilical vein. Some livers were additionally processed for scanning electron microscopy (SEM). The endogenous peroxidase was present in the nuclear envelope (NE) and endoplasmic reticulum (ER) of fetal macrophages with a negative reaction in the Golgi apparatus, a distribution pattern identical to that in Kupffer cells of adult rat liver. Such peroxidase-positive cells avidly took up the injected latex and carbon particles and were the only cell type in fetal liver involved in erythrophagocytosis. Furthermore, they were associated with erythropoietic elements, forming close contacts with such cells, especially normoblasts. The peroxidase pattern in leukopoietic cells differed at all stages of maturation from that in macrophages. By SEM the macrophages exhibited ruffles and lamellopodia on their surfaces and protruded often into the lumen of fetal sinusoids. Macrophages in fetal liver underwent mitotic divisions. The macrophages were first seen on gestation day 11, whereas the first mature monocytes were found on gestation day 17. These observations suggest that resident macrophages in fetal rat liver form a self-replicating cell line independent of the monocytopoietic series, although they may both arise from a common precursor cell.