PERIOPERATIVE PATTERN OF PERITONEAL INTERLEUKIN 8, TUMOUR NECROSIS FACTOR-α, AND GRANULOCYTE ELASTASE RELEASE IN HUMAN SECONDARY PERITONITIS

Abstract

Cytokine levels during infection and sepsis have been extensively studied in the past. In contrast to the excellent data on tumour necrosis factor α (TNF-α), interleukin 8 (IL-8), and polymorphonuclear (PMN) granulocyte elastase (PMN-E) concentrations in blood, little is known about cytokine and PMN-E levels in tissue or local fluids like abdominal exudate in secondary, purulent peritonitis of man. Therefore, the authors studied perioperative intra-abdominal levels of TNF-α, IL-8 and PMN-E in 21 patients with severe purulent peritonitis. The average pre-operative levels of TNF-α were 694+239 pg/ml in exudate and 26±6 pg/ml in plasma, for IL-8 100±34 ng/ml and 0.7±0.5 ng/ml, and for PMN-E 68±14 μg/ml and 0.7±0.1 μg/ml, respectively. Standard surgical procedures reduced the intra-abdominal concentrations of cytokines and PMN-E to as low as one tenth of the pre-operative levels. Postoperatively, TNF-α and IL-8 levels recovered rapidly and pre-operative levels of IL-8 were reached again after 1 h and for TNF-α after 8 h. PMN-E concentration remained below the initial baseline within 8 h of observation. TNF-α concentration, but not IL-8 or PMN-E, depended on the microbiological load of the abdominal exudate (< or >103cfu/ml). There were no significant differences in the intra-abdominal or plasma levels of cytokines or PMN-E between survivors and non-survivors at any observation time.