Abstract
Abstract Simultaneous quantification of cytokines is a powerful tool to identify associations between host immune defense and human immunodeficiency virus (HIV) pathogenesis. None of the commercially available cytokine detection methods however, have been rigorously validated in the context of HIV infection. Here we compared performance characteristics of two multiplex assays: the Meso Scale Discovery (MSD) platform and the Cytometric Bead Array (CBA) in specimens obtained from HIV-infected subjects. Overall, the MSD had a wider dynamic range and lower limits of quantification which lead to the ability to detect endogenous levels of serum cytokines that were assumed to not be present by CBA. In recombinant cytokine-spiked sera, MSD had better precision and accuracy based on intra-assay variability less than 25% and percent recovery within 25% of added concentrations. Regardless of the quantitation range, we observed variability between the analytes in both platforms with respect to the capacity to recover a known amount of cytokine. Nonetheless, the MSD platform facilitates the reliable assessment of cytokines present in low concentrations in the serum of HIV-infected individuals.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
