Abstract
Toxocariasis is one of zoonosis diseases that caused by Toxocara spp. that is Toxocara canis. Toxocara canis has several stages until it can infect animals and humans, namely the egg stage, larvae first stage (L1), larvae second stage (L2), larvae third stage (L3) to adult worms. Studies about the L2 and L2 tissue of T. canis found in paratenic hosts using Scanning Electron Microscopy (SEM) have not been widely performed. Some of the causes include L2 being not easily to found and identified, so research rarely raises the ultrastructural morphology of L2 and L2 tissues. Knowledge about the ultrastructural morphology of L2 and L2 tissue of T. canis worms is very important to determining the diagnosis, especially the etiological diagnosis. The purpose of this study is to detected morphology of L2 and L2 tissues of T. canis using SEM. Samples from this study is faeces of dogs that infected with toxocariasis and the digestive tract of dogs obtained from dog slaughter houses. The sample is an adult worm of T. canis; the female worm is dissected and taken uterus to collect worm eggs. The results of this study on microscopic and optilab examination showed a difference between L2 and L2 tissue that the length of L2 hatched from embryonic eggs was 390 ìm and with a width of 23.4 ìm at the midpoint of the body. Larvae second stage length from the infected somatic tissue is 410 ìm and the width is 22.5 ìm at the midpoint, and then difference in dorsal lip, cuticles, body ring, cervical alae, buccal capsul, tail.
Highlights
Toxocariasis is one of zoonosis diseases that caused by Toxocara spp. that is Toxocara canis
Studies about the L and L tissue of T. canis found in paratenic hosts using Scanning Electron Microscopy (SEM) have not been widely performed
Some of the causes include L2 being not to found and identified, so research rarely raises the ultrastructural morphology of L and L tissues
Summary
September 2019 Vol 20 No 3 : 390-396 dan L2 dari cacing T. canis sangat penting dalam menentukan diagnosis, terutama diagnosis etiologi. Tujuan dari penelitian ini adalah untuk mendeteksi morfologi ultrastruktur L dan L jaringan T. canis menggunakan SEM. Optilab menunjukkan perbedaan antara L2 dan L2 jaringan yaitu panjang L2 yang menetas dari telur adalah 390 ìm dan dengan lebar 23,4 ìm di titik tengah tubuh. Panjang larva stadium kedua dari jaringan somatik mencit yang diinfeksi adalah 410 ìm dan lebarnya 22,5 ìm di titik tengah, serta terdapat perbedaan pada dorsal lip, kutikula, body ring, cervical alae, buccal capsul, dan tail. Pengamatan terhadap berbagai stadium T. canis telah banyak dilaporkan terutama pada stadium telur dan dewasa dan dilakukan dengan beberapa cara seperti menggunakan scanning electron microscopy (SEM). Teknik SEM dapat digunakan untuk membandingkan morfologi ultrastruktur berbagai stadium larva dari cacing. L dan L jaringan T. canis yang terdapat pada inang paratenik menggunakan SEM masih belum banyak dilaporkan. Beberapa faktor penyebab antara lain karena L tidak mudah 2 ditemukan dan diidentifikasi sehingga jarang sekali penelitian yang mengangkat mengenai morfologi ultrastruktur L dan L jaringan
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