Abstract
The Role of Brush Border Membrane Vesicle (BBMV) in The Baculovirus Infection. Although baculovirus becoming recognized as potential important agents for control pests, there are several important obstacles for commercial use insecticide. One important obstacle is their narrow host. The other obstacle is due to its development resistance, the phenomenon whereby host larvae become progressively more resistant to fatal infection as they age within and among instars. From practical stand point, this phenomenon impacts the effectiveness of baculovirus control programs against agricultural and forest insect pests that is necessary to adjust application levels in the response to the demography of the target insect population. Purified columnar cells derived from midgut of armyworm Pseudaletia separata was used for fusion assay and observed by means of fluorescence assay based on self-quenching of octadecylrhodamine B chloride (R18). Confocal microscopy of the columnar cells that had been combined with labeled virus showed that the fluorescence appeared on the apical part in the brush border membrane, this indicated that there was fusion between virus and the cells. Two dimensional SDS-PAGE and immunobloting assay confirmed that the receptor exist within the columnar cells.
Highlights
Mem3hrmi bagrinxm suatu NIPS td6e\uk di dalamntl Bruloirns, n.nginfc*si nBB i rasi illeksi hinagr 'nLhi dd froses
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Berd.6-kn h^il SDS P,\GE dr lurarabkdiry Ai.6rkm rd6y2 resepbi \rEF Ai sinvili yrg bc+erd best d.l'm n.ngnjleg kd'bd!id" d:lnus$engturr'
Summary
Mem3hrmi bagrinxm suatu NIPS td6e\uk di dalamntl Bruloirns, n.nginfc*si nBB i rasi illeksi hinagr 'nLhi dd froses. -!\i;wiod, b:.,ddE-.ou;r@ftd" e .@d6 dimsiod SDs-?AcE sd ituoblddJ'g *ey 6!trh.d L\* th. D lElqati nsDs d.pa hi,gga 3dP.i usus @g2h sd,nggl us$ t6sih s.- $l,I sro k+.dum &s{ ber krur dengM pc.$unxu RN.dl.virus seL,ga bioPcstisidl xd,lah pcngcbhM Dasr L.n6g blologi Br.ulovins btk secda , /,n, mxupu i, uh, seru pe.geulrurn ckoloF Brculotus daho k ituva dengm smiFuhi lingkuAm
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