Abstract
We report here an approach of directional adsorption of purple membrane (PM) on liquid–solid interfaces modified by peptide assemblies. Bacteriorhodopsin (bR) is the only protein in PM that can transport protons directionally from the cytoplasmic (CP) side to the extracellular (EC) side to achieve chemical energy for life and growth. Controlled adsorption of PM is critical to exploring novel properties in many areas, such as data storage, biosolar devices, and sensors. Here, we obtained oriented PM adsorption at the liquid–solid interface by modification with de novo peptides. EFM was utilized to distinguish the two sides of PM by measuring the surface potential of PM because of its high resolution in differentiating electrical characteristics. Furthermore, we confirmed the modulating effect by photoelectrical responses under laser irradiation.
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