Abstract
Rat and human skin homogenate were fractionated using gel filtration of Sephadex G-100, and several enzymes hydrolysing dipeptide naphthylamides were partially separated. The presence of the following enzymes was demonstrated: 1. an enzyme hydrolysing glycyl-proline naphthylamide optimally at pH 7.6–7.8, corresponding to a similar enzyme found in the rat liver, 2. an enzyme hydrolysing lysyl-alanine naphthylamide optimally at pH 5.3–5.5, corresponding to the so-called dipeptidyl arylamidase II of bovine pituitary, 3. an sulfhydryl-dependent enzyme hydrolysing arginyl-arginine naphthylamide optimally at pH 8.0, corresponding to the so-called dipeptidyl arylamidase III of bovine pituitary, 4. a sulfhydryl-dependent enzyme hydrolysing alanyl-alanine naphthylamide optimally at pH 5.0, corresponding to cathepsin C. This enzyme was found to be present in the human skin only. In addition, aminopeptidase B, earlier demonstrated in the rat skin, was found to be present in the human skin.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Archiv f�r Klinische und Experimentelle Dermatologie
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
