Abstract
Dermatophytosis is one of the superficial mycoses which causes skin health problems in pet animals. This study conducted molecular characterization using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on specimens obtained from patients suspected of dermatophytosis in several clinics in DKI Jakarta Province and Bogor City. Fifty samples of skin scrapings from patients suspected of clinically dermatophytosis were collected and analyzed by conventional and molecular techniques. The Research aimed to identify dermatophyte that were isolated from pet animals using PCR-RFLP technique. The primers of ITS 1, ITS 4, Chytin Synthase, and cutting enzymes of Dde1 were used in this Research. Four specimens off 50 spesimens were tested positive using direct and culture PCR examination techniques. Based on the sequencing results, Microsporum canis was identified in four spesimens. Specimens that were positive for dermatophytes followed by RFLP using the Dde 1 enzyme. The results of the study showed that molecular techniques were a reliable way to determine the high-precision dermatophytes in diagnosing dermatophytosis. The Results also showed that molecular arrangement of B1 isolate was different from three other isolates.
Highlights
Dermatofitosis adalah salah satu mikosis superfisialis yang menyebabkan masalah kesehatan kulit pada hewan piara
This study conducted molecular characterization using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on specimens obtained from patients suspected of dermatophytosis in several clinics in DKI Jakarta Province and Bogor City
The primers of internal transcribed spacer (ITS) 1, ITS 4, Chytin Synthase, and cutting enzymes of Dde1 were used in this Research
Summary
PENDAHULUAN Dermatofitosis adalah mikosis superfisialis yang menyerang kulit, rambut dan kuku manusia dan hewan (Alshawa -et al, 2012). Dermatofitosis pada anjing dan kucing lebih sering disebabkan oleh kapang dermatofita Microsporum persicolor, M. gypseum, M. canis, dan Trichophyton sp. Infeksi yang disebabkan oleh M. canis dan Trichophyton mentagrophytes bersifat zoonotik dan hampir 20-50% dermatofitosis yang terjadi pada manusia disebabkan oleh kedua spesies tersebut. Teknik molekuler yang dapat digunakan untuk mengenali kapang dermatofita adalah teknik rantai reaksi polimerase (polymerase chain reaction/PCR) dan dilanjutkan dengan restriction fragment length polymorphism (RFLP) yang menyasar pada internal transcribed spacer regions (ITS). Teknik ini dapat mengenali dermatofita karena kemampuannya menghasilkan polimorfisme DNA khusus spesies dermatofita pada pola pita yang terlihat dari hasil pemeriksaan dengan elektroforesis jel agarosa (Mohammadi et al, 2015). Tujuan Penelitian ini adalah untuk untuk mengenali isolat kapang dermatofita yang diisolasi dari hewan kesayangan di Jakarta dan Bogor menggunakan PCR-RFLP dengan primer ITS 1, ITS 4, Chytin Synthase, dan enzim pemotong Dde
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