Abstract
Blood Disease Bacteria is one obstacle in cultivation of bananas. BDB can be controlled using biological agents. Biological agents that can be used to control of blood diseases are Pseudomonad fluorescent and microorganisms in EM4. Pseudomonad fluorescent used as biological agents because of its ability to produce antimicrobial compounds such as siderophores, antibiotics, volatile and cyanide. other than that, the EM4 microorganisms can produce antimicrobial against several bacterial pathogens. This study aims to determine the effect of combination Pseudomonad fluorescent and EM4 to inhibit the growth of BDB in vitro. This research was conducted in January 2016 in the Laboratory of Microbiology Department of Biology FMIPA UNP. Research using a completely randomized design (RAL) with 6 treatments and 3 replications. The study treatment is a combination of Pseudomonads fluorescent and EM4. The observation was done by measuring the clear zone formed around the paper disc. Data were analyzed using analysis of variance (ANOVA) and the test of Duncan New Multiple Range Test (DNMRT) at 5% level. The research proves that the combination resulted in inhibition zone around the paper disc. Inhibition zone shows that the combination inhibits the growth of BDB in vitro. Inhibition zone is formed as fluorescent Pseudomonads and microbes on EM4 produce secondary metabolites that can inhibit the growth of BDB.Key Word: Pseudomonad flourecent, BDB, EM4
Highlights
Pisang merupakan komoditas buah-buahan penting yang ada di Indonesia yang sangat mendukung ekonomi dan aktifitas budaya
This study aims to determine the effect of combination Pseudomonad fluorescent and EM4 to inhibit the growth of Blood Disease Bacteria (BDB) in vitro
B. Saran Disarankan untuk dilakukan penelitian lebih lanjut mengenai kombinasi pseudomonad flouresen dengan EM4 secara in planta sehingga dapat di terapkan oleh petani pisang dalam menangani penyakit Blood Disease Bacteria (BDB) yang menyerang tanaman pisang
Summary
Pisang merupakan komoditas buah-buahan penting yang ada di Indonesia yang sangat mendukung ekonomi dan aktifitas budaya. Penyakit pisang yang disebabkan oleh Blood Disease Bacteria (BDB) disebut juga dengan penyakit darah atau penyakit layu bakteri. Disease Bacteria) yang menyerang tanaman pisang secara in vitro.[6] Baharudin[12] melaporkan bahwa penggunaan Pseudomonas floresens dan Basilus subtilis yang ditambah dengan EM4 lebih efektif dalam mengendalikan penyakit layu bakteri pada tanaman kentang (Solanum tuberosum) 5 mm, kemudian kertas cakram dimasukkan ke dalam cawan petri dan disterilisasi dengan menggunakan autoclave dengan temperatur 121°C pada tekanan 15 psi selama 15 menit.
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