PD64-11 OVEREXPRESSION OF LNCRNA SUCLG2-AS1 INHIBITS CELL PROLIFERATION, MIGRATION AND INVASION THROUGH INHIBITING MAPK PATHWAY IN RENAL CELL CARCINOMA

Abstract

You have accessJournal of UrologyKidney Cancer: Basic Research & Pathophysiology III (PD64)1 Apr 2020PD64-11 OVEREXPRESSION OF LNCRNA SUCLG2-AS1 INHIBITS CELL PROLIFERATION, MIGRATION AND INVASION THROUGH INHIBITING MAPK PATHWAY IN RENAL CELL CARCINOMA Zhang Kenan*, Gong Yanqing, Cai Lin, and Gong Kan Zhang Kenan*Zhang Kenan* More articles by this author , Gong YanqingGong Yanqing More articles by this author , Cai LinCai Lin More articles by this author , and Gong KanGong Kan More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000981.011AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Pervious studies have revealed that long non-coding RNAs (lncRNAs) play an important role in renal cell carcinoma. However, the detailed roles of lncRNA SUCLG2-AS1 was rarely known and remained to be elucidated. METHODS: The genes of differential expression lncRNA were identified by TCGA.The relative expression level of SUCLG2-AS1 was determined by Real-Time qPCR in a total of 60 patients with clear cell renal cell carcinoma (ccRCC) and in different renal cell carcinoma (RCC) cell lines. Overexpression experiments were performed to investigate the biological roles of SUCLG2-AS1 on RCC cell proliferation, migration and invasion. Comprehensive transcriptional analysis, RNA-FISH and western blot were performed to explore the molecular mechanisms underlying the functions of SUCLG2-AS1. RESULTS: In this study, we found that SUCLG2-AS1 was downregulated in ccRCC tissues by analyzing TCGA online RNA-seq data. Moreover, decreased SUCLG2-AS1 expression level was positive associated with pathologic states and survival. Low expression of SUCLG2-AS1 was also confirmed in ccRCC tissues and RCC cell lines. In vitro, overexpression of SUCLG2-AS1 inhibits cell proliferation, migration and invasion in RCC cell lines. In a tumor xenograft model, we confirmed that SUCLG2-AS1 overexpression could significantly inhibit the growth of RCC. Mechanistically, we found that SUCLG2-AS1 was distributed mostly in the cytoplasm Furthermore, overexpression of SUCLG2-AS1 suppressed the expression of MAPK pathway related proteins in renal cell carcinoma. CONCLUSIONS: In conclusion, our findings demonstrated that SUCLG2-AS1 act as an suppressor gene in ccRCC, which may sever as a candidate prognostic biomarker for ccRCC patients. Source of Funding: This work was supported by the National Natural Science Foundation of China (grant 81572506), the Special Health Development Research Project of Capital (grant 2016-2-4074), and the Fundamental Research Funds for the Central Universities (grant BMU2018JI002). © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e1304-e1304 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Zhang Kenan* More articles by this author Gong Yanqing More articles by this author Cai Lin More articles by this author Gong Kan More articles by this author Expand All Advertisement PDF downloadLoading ...