PD21-10 AN EXPERIMENTAL STUDY OF TOPICAL INSULIN-LIKE GROWTH FACTOR-1 DELIVERY TO IMPROVE URETHRAL WOUND HEALING IN A RABBIT MODEL

Abstract

You have accessJournal of UrologyTrauma/Reconstruction/Diversion: Urethral Reconstruction (including Stricture, Diverticulum) I1 Apr 2018PD21-10 AN EXPERIMENTAL STUDY OF TOPICAL INSULIN-LIKE GROWTH FACTOR-1 DELIVERY TO IMPROVE URETHRAL WOUND HEALING IN A RABBIT MODEL Masayuki Shinchi, Toshihiro Kushibiki, Yoshine Mayumi, Keiichi Ito, Tomohiko Asano, Miya Ishihara, and Akio Horiguchi Masayuki ShinchiMasayuki Shinchi More articles by this author , Toshihiro KushibikiToshihiro Kushibiki More articles by this author , Yoshine MayumiYoshine Mayumi More articles by this author , Keiichi ItoKeiichi Ito More articles by this author , Tomohiko AsanoTomohiko Asano More articles by this author , Miya IshiharaMiya Ishihara More articles by this author , and Akio HoriguchiAkio Horiguchi More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.1165AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Urethral stricture is characterized by a narrowing of the urethra with formation of scar tissue, and few anti-fibrotic agents have been used to prevent urethral stricture formation after urethral injury. Insulin-like growth factor-1 (IGF-1) has emerged as an anti-fibrotic agent and we evaluated the effect of topical IGF-1 delivery on urethral wound healing after urethral injury. METHODS We made urethral catheters coated either with IGF-1 impregnated collagen hydrogel or with only collagen hydrogel. A total of 31 male Japanese white rabbits (2.5 to 3.0 kg) were divided into 3 groups after their urethras were injured by electrocoaglation using spherical monopolar electrocautery. Group 1 (n = 5, control group) received no additional treatment after urethral injury. Group 2 (n = 6, collagen hydrogel group) had a urethral catheter coated with only collagen hydrogel inserted immediately after urethral injury. Group 3 (n = 10, IGF-group) had a urethral catheter coated with IGF-1 impregnated collagen hydrogel inserted immediately after urethral injury. Fourteen days after urethral injury, retrograde urethrography and urethroscopy (diameter 2.7 mm) were performed to evaluate the injured urethra, and the injured segments were also histologically evaluated. RESULTS The mean urethral diameter on urethrography in group 3 (5.6 mm) was the largest among the three groups (1.9 mm in group 1 and 4.1 mm in group 2, p < 0.01, figure upper panel). The ratio of urethral lumen in injured urethra to that in normal urethra in group 3 (56.0%) was the largest among the 3 groups (21.0 % in group 1 and 37.6 % in group 2, p < 0.01, figure lower panel). Nine of 10 (90.0%) had a urethral lumen large enough for passage of a urethroscope, which is higher than the fraction in the other groups (1 of 5, 20.0%, in group 1 and 4 of 6, 66.7%, in group 2). The mean injured area not covered with urethral epithelium in group 3 (9.45 mm2) was the smallest among the three groups (36.26 mm2 in group 1 and 26.24 mm2 in group 2, p < 0.05). CONCLUSIONS Topical IGF-1 delivery with collagen hydrogel significantly improved urethral wound healing and prevented urethral narrowing. Thus it might be useful in the prevention of urethral stricture after internal urethrotomy or urethral dilation. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e471 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Masayuki Shinchi More articles by this author Toshihiro Kushibiki More articles by this author Yoshine Mayumi More articles by this author Keiichi Ito More articles by this author Tomohiko Asano More articles by this author Miya Ishihara More articles by this author Akio Horiguchi More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...