PD19-03 THE DELIVERY OF THE RECOMBINANT PROTEIN COCKTAIL IDENTIFIED BY STEM CELL-DERIVED SECRETOME ANALYSIS ACCELERATES KIDNEY REPAIR AFTER RENAL ISCHEMIA-REPERFUSION INJURY

Abstract

You have accessJournal of UrologyCME1 Apr 2023PD19-03 THE DELIVERY OF THE RECOMBINANT PROTEIN COCKTAIL IDENTIFIED BY STEM CELL-DERIVED SECRETOME ANALYSIS ACCELERATES KIDNEY REPAIR AFTER RENAL ISCHEMIA-REPERFUSION INJURY Ji Hyun Kim, Heejo Yang, Michael Kim, Kang Su Cho, Doo Sang Kim, Hyung Eun Yim, Zachary Atala, John Jackson, In Kap Ko, and James Yoo Ji Hyun KimJi Hyun Kim More articles by this author , Heejo YangHeejo Yang More articles by this author , Michael KimMichael Kim More articles by this author , Kang Su ChoKang Su Cho More articles by this author , Doo Sang KimDoo Sang Kim More articles by this author , Hyung Eun YimHyung Eun Yim More articles by this author , Zachary AtalaZachary Atala More articles by this author , John JacksonJohn Jackson More articles by this author , In Kap KoIn Kap Ko More articles by this author , and James YooJames Yoo More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003285.03AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Renal disease is a worldwide health issue. Stem cell therapies have been proposed to restore damaged kidneys as an alternative to renal replacement therapies. In addition to stem cell therapies, recent studies have shown that stem cell-derived secretomes or conditioned media (CM) can enhance tissue regeneration. Our previous study demonstrated that the controlled delivery of human placental stem cells (hPSC)-derived CM using platelet-rich plasma (PRP) improved histological and functional recovery in an acute kidney injury (AKI) model in rats. The protein analysis of hPSC-CM showed the five highly expressed proteins that promote kidney repair. Based on our results, we propose that these proteins could be used as a recombinant protein cocktail to treat kidney diseases as an alternative to using CM. This study investigated the feasibility of delivering the recombinant protein cocktail for kidney repair after AKI. METHODS: The effects of the recombinant protein cocktail on human renal cell survival, proliferation, and apoptosis were compared with the CM in vitro. The feasibility of delivering the recombinant protein cocktail with a PRP system to achieve structural and functional recovery after AKI was investigated. RESULTS: The pro-proliferative and anti-apoptotic effects of the protein cocktail on renal cells were demonstrated in vitro and in vivo. The intrarenal delivery of these proteins with PRP ameliorates the renal tubular damage and improved renal function in the AKI-induced rats, yielding similar therapeutic effects compared to the CM delivery. CONCLUSIONS: The delivery of RPC can support renal cell survival and proliferation in vitro and in vivo, resulting in the attenuation of renal tubular damages and amelioration of renal function in the AKI model in rats. Our strategy may provide a therapeutic solution to many challenges associated with kidney repair resulting from the lack of suitable off-the-shelf regenerative medicine products. Source of Funding: This work was supported by the State of North Carolina © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e577 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Ji Hyun Kim More articles by this author Heejo Yang More articles by this author Michael Kim More articles by this author Kang Su Cho More articles by this author Doo Sang Kim More articles by this author Hyung Eun Yim More articles by this author Zachary Atala More articles by this author John Jackson More articles by this author In Kap Ko More articles by this author James Yoo More articles by this author Expand All Advertisement PDF downloadLoading ...