Abstract
The quality of drinking water has an important role in human health. This study was aimed to detect Escherichia. coli, Salmonella sp. and Vibrio cholerae from bottled drinking waters produced in Iran. A total of 240 samples of bottled water of different brands were collected for testing between March 2015 to December 2015 in Shahrekord-Iran. Samples were examined by polymerase chain reaction (PCR) combined with culture methods for the detection of E. coli, Salmonella sp., and V. cholerae. The results of PCR revealed that the uidA gene from E. coli, IpaB gene from Salmonella sp, and epsM gene from V. cholerae were detected in 6 (2.5%), 1 (0.4 %), 0 (0%) of the samples, respectively. But in culture methods, only E. coli 5 (2.1%) were isolated from the samples. The contamination with E. coli was significantly higher (P < 0.05) in water produced during the hot seasons than the cold seasons. This study confirmed the presence of Escherichia coli as the main microorganism in bottle drinking water in Iran. Also, our study showed that PCR can be used as a screening method for monitoring the enteric pathogens in drinking water.
Highlights
The quality of drinking water has an important role in human health
The results of the polymerase chain reaction (PCR) techniques showed that the specific uidA, IpaB, and epsM gene targets of E. coli, Salmonella sp., and V. cholerae were detected in 6 (2.5%), 1(0.4%), and 0 (0%) of 240 bottle drinking water samples, respectively
The results of the culture of the samples revealed that five (2.1 %) samples were contaminated with E. coli, but Salmonella sp. and V. cholerae were not culture positive from the samples (Table 2)
Summary
The quality of drinking water has an important role in human health. This study was aimed to detect Escherichia. coli, Salmonella sp. and Vibrio cholerae from bottled drinking waters produced in Iran. Vibrio cholerae from bottled drinking waters produced in Iran. Samples were examined by polymerase chain reaction (PCR) combined with culture methods for the detection of E. coli, Salmonella sp., and V. cholerae. Results: The results of PCR revealed that the uidA gene from E. coli, IpaB gene from Salmonella sp, and epsM gene from V. cholerae were detected in 6 (2.5%), 1 (0.4 %), 0 (0%) of the samples, respectively. Conclusions: This study confirmed the presence of Escherichia coli as the main microorganism in bottle drinking water in Iran. Our study showed that PCR can be used as a screening method for monitoring the enteric pathogens in drinking water. One of the means of satisfying the need for portable water especially in urban communities is to consume packaged water which in Iran is sold in plastic bottles. Apart from microbiological considerations, the upsurge in the demand for bottled water has prompted the interest of many manufacturers in the production of bottled water
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