Abstract
Currently tuberculosis is considered as a group of diseases united by one etiological factor. The pathogenesis of certain localizations of tuberculous inflammation, in particular peritoneum tuberculosis, hasn’t been sufficiently studied. The role of cytokine mechanisms in the development of the disease and the elaboration of non-sterile immunity requires further experimental studies, in particular the creation of a reproducible model on laboratory animals.The aim: to study the effect of TNF-α on the development of tuberculosis of the serous coat of the abdominal cavity, as well as to evaluate the possibility of modeling tuberculous peritonitis in laboratory animals using infliximab.Materials and methods. The studies were conducted on 18 male rabbits, which were simulated peritoneal tuberculosis by intra-abdominal administration of a suspension of Mycobacterium tuberculosis. 10 rabbits of the experimental group were intravenously injected with an infliximab solution and an iron (III) hydroxide sucrose complex intraperitoneally a day before infection.Results. In the control group of animals, tuberculosis either didn’t develop, or in a third of cases it affected only the pulmonary parenchyma, while proliferative processes prevailed. On the contrary, in animals with inactivated TNF-α, in 100 % of observations, tuberculous peritonitis was detected with associated lung damage and the predominance of alterative caseous processes.Conclusion. The created model of tuberculous peritonitis shows the leading role of TNF-α in the activation of macrophages, as well as in attracting cells to the site of infection. This is the primary signal necessary for the formation and stability of granulomas since the neutralization of this cytokine leads to a loss of control over the infection and the destruction of the granuloma with the development of destructive tuberculosis in the serous coat of the abdominal cavity.
Highlights
ФТ И З И АТ Р И Я PHTHISIOLOGYПАТОГЕНЕТИЧЕСКАЯ РОЛЬ ФАКТОРА НЕКРОЗА ОПУХОЛЕЙ (TNF-α) ДЛЯ РАЗВИТИЯ ТУБЕРКУЛЁЗА БРЮШИНЫ В ЭКСПЕРИМЕНТЕ
Проблема борьбы с туберкулёзом побуждает исследователей к дальнейшему изучению патогенетических особенностей этой древнейшей инфекции и иммунологических механизмов, направленных на элиминацию патогена из организма
The absence of a fibrous capsule on the edge of the focus of caseous necrosis; a – staining with hematoxylin and eosin, б, в – Van Gieson staining (а – ×100, б – ×40, в – ×200)
Summary
ПАТОГЕНЕТИЧЕСКАЯ РОЛЬ ФАКТОРА НЕКРОЗА ОПУХОЛЕЙ (TNF-α) ДЛЯ РАЗВИТИЯ ТУБЕРКУЛЁЗА БРЮШИНЫ В ЭКСПЕРИМЕНТЕ. Патогенез отдельных локализаций туберкулёзного воспаления, в частности туберкулёз брюшины, ещё недостаточно изучен. Цель исследования: изучить влияние TNF-α на развитие туберкулёза серозного покрова брюшной полости, а также оценить возможность моделирования туберкулёзного перитонита у лабораторных животных с помощью инфликсимаба. Исследования проведены на 18 кроликах-самцах, которым моделировали туберкулёз брюшины с помощью внутрибрюшного введения суспензии Mycobacterium tuberculosis. Созданная модель туберкулёзного перитонита показывает ведущую роль TNF-α в активации макрофагов, а также в привлечении клеток к месту инфекции. Ключевые слова: туберкулёзный перитонит, кролик, фактор некроза опухолей, модель туберкулёзного перитонита, инфликсимаб. Для цитирования: Плоткин Д.В., Виноградова Т.И., Решетников М.Н., Зюзя Ю.Р., Оковитый С.В., Синицын М.В., Гайтукаев В.Р., Родоман Г.В., Богородская Е.М., Яблонский П.К. Патогенетическая роль фактора некроза опухолей (TNF-α) для развития туберкулёза брюшины в эксперименте. Plotkin D.V. 1, 2, Vinogradova T.I. 3, Reshetnikov M.N. 1, Zyuzya Yu.R. 1, Okovityi S.V. 4, Sinitsyn M.V. 1, Gaitukaev V.R. 5, Rodoman G.V. 2, Bogorodskaya E.M. 1, Yablonskiy P.K. 3
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