Abstract
Objective To investigate the pathogen of an aseptic meningitis outbreak in Linfen city, Shanxi province. Methods Real-time PCR was used to screen enterovirus among 9 throat swab samples. The genotyping of the enterovirus was identifed by PCR and sequencing of 5’UTR region and VP2 gene. The sequence and phylogenetic analysis of the VP1 genes of ECHO30 was performed using the MEGA 4.0 software. Results Four out of 9 samples were identified as enterovirus positive by real-time PCR. Phylogenetic analysis of VP1 genes demonstrated that 4 strains detected from this outbreak were aggregated into one cluster, with the variability of 0.5%, and the closest relationships with them were ECHO30 isolated in 2004 from Zhejiang, China and with high homology with ECHO30 prototype, Bastianni strain (>75%). Conclusions ECHO30 is the predominant type of the aseptic meningitis outbreak in Linfen city. It is necessary to monitor pathogen spectrum associated with aseptic meningitis in this region. Key words: Aseptic meningitis; Enterovirus; Phylogenetic analysis
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