Abstract
It is the aim of this study to determine the influence of substratum surface free energy, protein concentration, adsorption time, shear rate and pH on the distribution patterns of adsorbed fibronectin films on FEP‐Teflon, PMMA and glass. Replicas of the adsorbed protein surface were made and examined by transmission electron microscopy. On high energy substrata, adsorption occurred in a grained reticulated manner, whereas on low energy substrata adsorption occurred in a patchy way. At low shear rates, fibronectin distribution patterns became more irregular at higher protein concentrations and complete surface coverage occurred on FEP‐Teflon, whereas at a higher shear rate, an inverse effect of protein concentration was seen. At pH 4–8, a pH close to the iso‐electric point of fibronectin, the fine reticulated structures on glass and PMMA, observed at pH 70, turned into more coarsely knotted structures, whereas on FEP‐Teflon structures became more patchy, indicative of a more extensive unfolding of the prot...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
